Volpato Lusiane, Gabardo Marilisa Carneiro Leão, Leonardi Denise Piotto, Tomazinho Paulo Henrique, Maranho Leila Teresinha, Baratto-Filho Flares
School of Health and Biological Sciences, Positivo University, Rua Prof. Pedro Viriato Parigot de Souza 5300, Curitiba, Paraná, 81280-330, Brazil.
BMC Res Notes. 2017 Mar 6;10(1):119. doi: 10.1186/s13104-017-2443-x.
Persea major Kopp (Lauraceae) is a plant with wound healing, antibacterial, and analgesic properties. The aim of this study was to assess the in vitro antibacterial activity of the concentrated crude extract (CCE) and ethyl acetate fraction (EAF) of this plant against Enterococcus faecalis and compare it with calcium hydroxide [Ca(OH)] paste and 2% chlorhexidine digluconate (CHX).
The plant material was collected, and an extract was prepared according to the requirements of the study (CCE and EAF). The minimum inhibitory concentrations (MICs) of CCE, EAF, Ca(OH), Ca(OH) + CCE, and CHX against E. faecalis were determined using the broth microdilution method RESULTS: The EAF inhibited E. faecalis at concentrations of 166.50, 83.25, and 41.62 mg mL, and 1.00, 0.50, and 0.25% of CHX solutions showed antimicrobial activity. The MICs of Ca(OH) paste were 166.50 and 83.25 mg mL, whereas Ca(OH) + CCE showed antimicrobial activity only at a concentration of 166.50 mg mL. CCE showed no inhibitory effect at any of the concentrations tested CONCLUSIONS: The CCE did not show any antimicrobial activity against E. faecalis; however, the EAF was the most effective among the three highest concentrations tested.
大鳄梨（樟科）是一种具有伤口愈合、抗菌和止痛特性的植物。本研究的目的是评估该植物的浓缩粗提物（CCE）和乙酸乙酯部位（EAF）对粪肠球菌的体外抗菌活性，并将其与氢氧化钙[Ca(OH)]糊剂和2%葡萄糖酸氯己定（CHX）进行比较。
采集植物材料，并根据研究要求制备提取物（CCE和EAF）。采用肉汤微量稀释法测定CCE、EAF、Ca(OH)、Ca(OH)+CCE和CHX对粪肠球菌的最低抑菌浓度（MICs）。结果：EAF在浓度为166.50、83.25和41.62mg/mL时可抑制粪肠球菌，1.00%、0.50%和0.25%的CHX溶液具有抗菌活性。Ca(OH)糊剂的MICs为166.50和83.25mg/mL，而Ca(OH)+CCE仅在浓度为166.50mg/mL时具有抗菌活性。CCE在任何测试浓度下均未显示出抑制作用。结论：CCE对粪肠球菌未显示出任何抗菌活性；然而，在测试的三个最高浓度中，EAF是最有效的。
