Lu Qing-Li, Liu Jian, Zhu Xiao-Li, Xu Wen-Jia
Department of Geriatrics, Huazhong University of Science and Technology, Wuhan, 430030, China.
Huazhong University of Science and Technology, Wuhan, 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2014 Jun;34(3):359-362. doi: 10.1007/s11596-014-1283-3. Epub 2014 Jun 18.
In order to investigate the expression of nerve growth factor (NGF) and hypoxia inducible factor-1α (HIF-1α) and its correlation with angiogenesis in non-small cell lung cancer (NSCLC), paraffin-embedded tissue blocks from 20 patients with NSCLC were examined. Twenty corresponding para-cancerous lung tissue specimens were obtained to serve as a control. The expression of NGF, HIF-1α, and vascular endothelial growth factor (VEGF) in the NSCLC tissues was detected by using immunohistochemistry. The microvascular density (MVD) was determined by CD31 staining. The results showed that the expression levels of NGF, HIF-1α and VEGF in the NSCLC tissues were remarkably higher than those in the para-cancerous lung tissues (P<0.05). There was significant difference in the MVD between the NSCLC tissues (9.19±1.43) and para-cancerous lung tissues (2.23±1.19) (P<0.05). There were positive correlations between NGF and VEGF, between HIF-1α and VEGF, and between NGF and HIF-1α in NSCLC tissues, with the spearman correlation coefficient being 0.588, 0.519 and 0.588, respectively. In NSCLC tissues, the MVD had a positive correlation with the three factors (P<0.05). Theses results suggest that NGF and HIF-1α are synergically involved in the angiogenesis of NSCLC.
为了研究神经生长因子（NGF）和缺氧诱导因子-1α（HIF-1α）的表达及其与非小细胞肺癌（NSCLC）血管生成的相关性，对20例NSCLC患者的石蜡包埋组织块进行了检测。获取20例相应的癌旁肺组织标本作为对照。采用免疫组织化学法检测NSCLC组织中NGF、HIF-1α和血管内皮生长因子（VEGF）的表达。通过CD31染色测定微血管密度（MVD）。结果显示，NSCLC组织中NGF、HIF-1α和VEGF的表达水平明显高于癌旁肺组织（P<0.05）。NSCLC组织（9.19±1.43）与癌旁肺组织（2.23±1.19）的MVD有显著差异（P<0.05）。NSCLC组织中NGF与VEGF、HIF-1α与VEGF、NGF与HIF-1α之间均呈正相关，Spearman相关系数分别为0.588、0.519和0.588。在NSCLC组织中，MVD与这三个因子均呈正相关（P<0.05）。这些结果表明，NGF和HIF-1α协同参与了NSCLC的血管生成。
