Omidbakhsh Navid, Ahmadpour Faraz, Kenny Nicole
Research & Development, Virox Technologies, Inc., Oakville, Ontario, Canada.
Professional & Technical Services, Virox Technologies Inc., Oakville, Ontario, Canada.
PLoS One. 2014 Jun 18;9(6):e99951. doi: 10.1371/journal.pone.0099951. eCollection 2014.
Meters based on adenosine triphosphate (ATP) bioluminescence measurements in relative light units (RLU) are often used to rapidly assess the level of cleanliness of environmental surfaces in healthcare and other settings. Can such ATP measurements be adversely affected by factors such as soil and cleaner-disinfectant chemistry?
This study tested a number of leading ATP meters for their sensitivity, linearity of the measurements, correlation of the readings to the actual microbial contamination, and the potential disinfectant chemicals' interference in their readings.
First, solutions of pure ATP in various concentrations were used to construct a standard curve and determine linearity and sensitivity. Serial dilutions of a broth culture of Staphylococcus aureus, as a representative nosocomial pathogen, were then used to determine if a given meter's ATP readings correlated with the actual CFUs. Next, various types of disinfectant chemistries were tested for their potential to interfere with the standard ATP readings.
All four ATP meters tested herein demonstrated acceptable linearity and repeatability in their readings. However, there were significant differences in their sensitivity to detect the levels of viable microorganisms on experimentally contaminated surfaces. Further, most disinfectant chemistries tested here quenched the ATP readings variably in different ATP meters evaluated.
Apart from their limited sensitivity in detecting low levels of microbial contamination, the ATP meters tested were also prone to interference by different disinfectant chemistries.
基于三磷酸腺苷（ATP）生物发光测量相对光单位（RLU）的仪器常用于快速评估医疗保健及其他场所环境表面的清洁程度。诸如污垢和清洁剂 - 消毒剂化学成分等因素会对这种ATP测量产生不利影响吗？
本研究测试了多款领先的ATP仪器，考察其灵敏度、测量的线性度、读数与实际微生物污染的相关性以及潜在消毒化学品对其读数的干扰情况。
首先，使用不同浓度的纯ATP溶液构建标准曲线并确定线性度和灵敏度。然后，对作为代表性医院病原体的金黄色葡萄球菌肉汤培养物进行系列稀释，以确定给定仪器的ATP读数是否与实际菌落形成单位（CFU）相关。接下来，测试了各种类型的消毒化学成分干扰标准ATP读数的可能性。
本文测试的所有四款ATP仪器在其读数中均显示出可接受的线性度和重复性。然而，它们在检测实验污染表面上活微生物水平的灵敏度方面存在显著差异。此外，这里测试的大多数消毒化学成分在不同评估的ATP仪器中对ATP读数的淬灭程度各不相同。
除了在检测低水平微生物污染方面灵敏度有限外，所测试的ATP仪器还容易受到不同消毒化学成分的干扰。
