Xu Baojun, Li Chantian, Sung Changkeun
Food Science and Technology Program, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai, China.
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Changchun, China.
Int J Med Mushrooms. 2014;16(1):17-28. doi: 10.1615/intjmedmushr.v16.i1.20.
Telomerase has been widely accepted as a cancer marker and a promising therapeutic target for novel anticancer drugs. The aim of this study was to investigate the in vitro telomerase inhibitory effects of mushrooms and their anticancer properties. The inhibitory effects of mushrooms and lichens against telomerase activity of HL-60 cells were systematically assessed using polymerase chain reaction based on assay of telomeric repeat amplification protocol. Telomerase inhibitory samples were further tested for antiproliferation effects against the gastric cell line SNU-1 using the MTT method. Ethyl acetate extract of Pleurotus ostreatus, ethyl acetate and water extracts of Lasiosphaera fenzlii, hexane extract of Strobilomyces floccopus, water extract of Sarcodon aspratus, and hexane, ethyl acetate, and water extracts from Umbilicaria esculenta showed strong positive telomerase inhibitory activity. Hexane extract of S. floccopus and water extracts from the edible lichen U. esculenta exhibited strong anticancer effects against SNU-1 cells through antiproliferation assay. The water extract of U. esculenta has a great potential to be developed into an anticancer agent that targets telomerase.
端粒酶已被广泛认可为一种癌症标志物和新型抗癌药物的有前景的治疗靶点。本研究的目的是研究蘑菇的体外端粒酶抑制作用及其抗癌特性。基于端粒重复序列扩增协议分析，采用聚合酶链反应系统评估了蘑菇和地衣对HL-60细胞端粒酶活性的抑制作用。使用MTT法进一步测试端粒酶抑制样品对胃细胞系SNU-1的抗增殖作用。平菇乙酸乙酯提取物、脱皮马勃乙酸乙酯和水提取物、毛头鬼伞己烷提取物、糙皮侧耳水提取物以及脐衣己烷、乙酸乙酯和水提取物均表现出较强的端粒酶抑制活性。通过抗增殖试验，毛头鬼伞己烷提取物和可食用地衣脐衣的水提取物对SNU-1细胞表现出较强的抗癌作用。脐衣水提取物具有很大的潜力被开发成一种靶向端粒酶的抗癌药物。
