Reza Md Ahsanur, Hossain Md Akil, Lee Seung-Jin, Yohannes Sileshi Belew, Damte Dereje, Rhee Man-hee, Jo Woo-Sik, Suh Joo-Won, Park Seung-Chun
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Kyungpook National University, Daegu 702701, Republic of Korea.
College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
Int J Med Mushrooms. 2014;16(1):37-47. doi: 10.1615/intjmedmushr.v16.i1.40.
In this study, a dichloromethane fraction (DCMF) from 70% Auricularia auricula-judae ethanol extract showed the highest level of antitumor activity compared to other solvent fractions (ethyl acetate, butanol, and water). The DCMF was found to have more potent antitumor activity against broncheoalveolar cancer (half maximal inhibitory concentration = 57.2 µg/mL) and gastric cancer cells (half maximal inhibitory concentration = 73.2 µg/mL) compared to the other solvent fractions, although all fractions inhibited the proliferation of the tumor cells in a dose-dependent manner. We further analyzed the DCMF composition by gas chromatography-coupled mass spectroscopy. Based on the results of this analysis, an antitumor active component (diazane) was identified in the DCMF. However, we found that diazane alone had a lower level of antitumor activity than the DCMF. These findings indicate that other unknown components of the DCMF also are responsible for the cytotoxic effects of DCMF against tumor cells. Semiquantitative reverse transcription polymerase chain reaction analysis demonstrated that DCMF induced cytotoxicity or tumor cell apoptosis as a result of the downregulation of Bcl-2 expression and p53 overexpression. Taken together, our study results demonstrated that the DCMF may be used as a functional additive for enhancing antioxidant activities and suppressing tumor growth in the body.
在本研究中，与其他溶剂萃取物（乙酸乙酯、丁醇和水）相比，70%木耳乙醇提取物的二氯甲烷萃取物（DCMF）显示出最高水平的抗肿瘤活性。发现DCMF对支气管肺泡癌（半数最大抑制浓度 = 57.2 µg/mL）和胃癌细胞（半数最大抑制浓度 = 73.2 µg/mL）具有比其他溶剂萃取物更强的抗肿瘤活性，尽管所有萃取物均以剂量依赖方式抑制肿瘤细胞的增殖。我们通过气相色谱 - 质谱联用进一步分析了DCMF的成分。基于该分析结果，在DCMF中鉴定出一种抗肿瘤活性成分（二氮烷）。然而，我们发现单独的二氮烷抗肿瘤活性水平低于DCMF。这些发现表明，DCMF的其他未知成分也对DCMF对肿瘤细胞的细胞毒性作用负责。半定量逆转录聚合酶链反应分析表明，DCMF由于Bcl - 2表达下调和p53过表达而诱导细胞毒性或肿瘤细胞凋亡。综上所述，我们的研究结果表明，DCMF可用作功能性添加剂，以增强抗氧化活性并抑制体内肿瘤生长。
