Faculty of Traditional Thai Medicine, Prince of Songkla University , Hat-Yai, Songkhla 90112, Thailand.
J Nat Prod. 2014 Jul 25;77(7):1562-71. doi: 10.1021/np400996k. Epub 2014 Jun 18.
Two rare new natural products, the neocaged-xanthone pruniflorone T (1) and the rearranged caged-xanthone pruniflorone U (3), and the known caged-xanthone cochinchinone C (2) were isolated from the roots of Cratoxylum formosum ssp. pruniflorum. The unique structures of 1-3 were determined by analysis of NMR and X-ray diffraction data. The X-ray data of 1-3 revealed that they all exist with both enantiomers in their crystal packing. Separation of 1-3 by chiral HPLC led to the isolation of three pairs of enantiomers, (-)-1/(+)-1, (-)-2/(+)-2, and (-)-3/(+)-3, and their absolute configurations were determined by analysis of single-crystal X-ray diffraction and ECD spectroscopic data. A 1:1 mixture of 1 and 3 showed potent in vitro cytotoxicity against an MCF-7 human breast cancer cell line with an IC50 value of 0.11 μg/mL.
从红花天料木的根部分离得到两个新的罕见天然产物，即新笼状黄烷酮普瑞福酮 T（1）和重排笼状黄烷酮普瑞福酮 U（3），以及已知的笼状黄烷酮交让木酮 C（2）。通过 NMR 和 X 射线衍射数据分析确定了 1-3 的独特结构。X 射线数据表明，它们在晶体堆积中都以两种对映异构体的形式存在。通过手性 HPLC 对 1-3 进行分离，得到了三对对映异构体（-）-1/（+）-1、（-）-2/（+）-2 和（-）-3/（+）-3，并通过单晶 X 射线衍射和 ECD 光谱数据分析确定了它们的绝对构型。1 和 3 的 1:1 混合物对 MCF-7 人乳腺癌细胞系表现出强烈的体外细胞毒性，IC50 值为 0.11 μg/mL。
