Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Human High Performance and Health Promotion Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand.
Molecules. 2021 Jul 12;26(14):4235. doi: 10.3390/molecules26144235.
ssp. (Kurz) Gogelein (CP) is an indigenous plant found mainly in southeast Asia. Several in vitro studies have confirmed its activity against hepatocellular carcinoma; however, in vivo studies of the effect of CP on liver cancer are needed. This study investigated the effect of CP on early-stage hepatocarcinogenesis in rat liver when using diethylnitrosamine (DEN) as a carcinogen. Immunohistochemistry was used to detect (a) upregulation of glutathione -transferase placental (GST-P) positive foci, (b) the proliferating cell nuclear antigen PCNA, and (c) apoptotic cells in the liver as indicators of early-stage carcinogenesis. Immunohistochemical parameters were observed in rats given CP orally following DEN injection. Rats given DEN presented overexpression of GST-P positive foci, PCNA, and apoptotic cells, indicating the formation of cancerous tissues, and these effects were diminished by CP treatment. CP thus inhibited hepatocarcinogenic effects in an animal model. These results could help plan further in vivo studies and support the use of CP to prevent processes that promote the pathogenesis of hepatocellular carcinoma in humans.
ssp. (Kurz) Gogelein (CP) 是一种主要存在于东南亚的本土植物。几项体外研究已经证实了它对肝癌细胞的活性；然而，需要进行 CP 对肝癌的体内研究。本研究以二乙基亚硝胺 (DEN) 作为致癌剂，研究了 CP 对大鼠肝早期肝癌发生的影响。免疫组织化学用于检测 (a) 谷胱甘肽-S-转移酶胎盘型 (GST-P) 阳性焦点的上调，(b) 增殖细胞核抗原 PCNA，以及 (c) 作为早期致癌作用的肝中的凋亡细胞。观察了 DEN 注射后给予 CP 口服的大鼠中的免疫组织化学参数。给予 DEN 的大鼠 GST-P 阳性焦点、PCNA 和凋亡细胞过度表达，表明癌组织的形成，而 CP 处理减轻了这些作用。因此，CP 在动物模型中抑制了肝癌的致癌作用。这些结果有助于进一步计划体内研究，并支持使用 CP 来预防促进人类肝细胞癌发病机制的过程。
