Wang Yun, Chen Zhang-jian, Ba Te, Pu Ji, Cui Xiao-xing, Jia Guang
Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2014 Jun 18;46(3):395-9.
To compare the effect of TiO₂ nanoparticles on antioxidant function and element content of liver and kidney tissues in young and adult rats.
Forty-eight SD male rats, half in 4-week (youth) old and half in 9-week (adult) old rats, were randomly divided into 8 groups, which were exposed to TiO₂ nanoparticles [(75 ± 15) nm, anatase] through intragastric administration at 0, 10, 50 and 200 mg/kg body weight every day for 30 days. The liver and kidney tissues were collected for antioxidant function and element content analysis.
200 mg/kg TiO₂ nanoparticles exposure significantly increased the liver total superoxide dismutase (T-SOD) activity and the kidney reduced glutathione (GSH)/oxidized glutathione (GSSG) ratios in young rats, and significantly decreased the liver Mo, Co, Mn and P contents and the kidney Rb and Na contents in young rats. 200 mg/kg TiO₂ nanoparticles exposure significantly increased GSH/GSSG ratios and Rb contents and decreased Na contents in the liver of adult rats. No significantly difference was found in antioxidant indexes and elements content in the kidney of adult rats between three experimental groups and control group.
TiO₂ nanoparticles can enhance the antioxidant capacity and decrease the elements content in rat liver and kidney tissues. The liver is the more sensitive target organ and the young animals are more susceptible to TiO₂ nanoparticles toxicity by the oral routes.
比较二氧化钛纳米颗粒对幼年和成年大鼠肝脏及肾脏组织抗氧化功能和元素含量的影响。
48只雄性SD大鼠，一半为4周龄（幼年），一半为9周龄（成年），随机分为8组，每天经口给予二氧化钛纳米颗粒[（75±15）nm，锐钛矿型]，剂量分别为0、10、50和200mg/kg体重，持续30天。收集肝脏和肾脏组织进行抗氧化功能和元素含量分析。
200mg/kg二氧化钛纳米颗粒暴露显著提高了幼年大鼠肝脏总超氧化物歧化酶（T-SOD）活性和肾脏还原型谷胱甘肽（GSH）/氧化型谷胱甘肽（GSSG）比值，并显著降低了幼年大鼠肝脏中钼、钴、锰和磷的含量以及肾脏中铷和钠的含量。200mg/kg二氧化钛纳米颗粒暴露显著提高了成年大鼠肝脏中GSH/GSSG比值和铷含量，并降低了钠含量。成年大鼠肾脏中抗氧化指标和元素含量在三个实验组与对照组之间未发现显著差异。
二氧化钛纳米颗粒可增强大鼠肝脏和肾脏组织的抗氧化能力并降低元素含量。肝脏是更敏感的靶器官，幼年动物经口途径对二氧化钛纳米颗粒毒性更敏感。
