Heimlich J Brett, Pollock David M
Department of Physiology, Georgia Regents University, Augusta, Georgia, USA.
Cardio-Renal Physiology and Medicine, Division of Nephrology, Department of Medicine, The University of Alabama at Birmingham, Birmingham, Alabama, USA.
Physiol Rep. 2014 Jun 18;2(6):e12052. doi: 10.14814/phy2.12052.
Previous studies from our laboratory report variation in nitric oxide (NO)-dependent arterial pressure within the same strain of normotensive Sprague-Dawley rat dependent upon the commercial vendor supplying the rats. Clinical assessment of endothelial NO activity and endothelial function in general has used postocclusion, flow-mediated dilation (FMD). Therefore, this study was conducted to determine whether the reactive hyperemic response was different between two normotensive strains from two different suppliers, Sprague-Dawley (SD) and Wistar-Kyoto (WKY) rats from Charles River (CR) and Harlan Laboratories (H), respectively. Rats were anesthetized and the femoral artery was occluded for 5 min, with femoral blood flow measured continuously by use of an ultrasonic perivascular flow probe. The average area under the reactive hyperemic response curve (3-min duration) was not different between SD rats from CR (80 ± 23 mL/min∙s; n = 6) and H (94 ± 16 mL/min∙s; n = 6). As previously reported, blood pressures were higher in the SD rats from H versus CR. WKY rats from both suppliers had significantly larger hyperemia; 371 ± 67 versus 281 ± 71 mL/min∙s (n = 5) for the CR and H WKY rats, respectively, but again, were not different between vendors. Blood pressures in WKY rats were similar between vendors. These results suggest that differences in NO bioactivity are not discernable with an adapted FMD protocol in the rat and that normotensive strains of rat can have large differences in reactive hyperemia despite having similar blood pressures.
我们实验室之前的研究报告称，在同一品系的正常血压Sprague-Dawley大鼠中，一氧化氮（NO）依赖性动脉压存在差异，这取决于供应大鼠的商业供应商。一般而言，内皮NO活性和内皮功能的临床评估采用闭塞后血流介导的血管舒张（FMD）。因此，本研究旨在确定分别来自两个不同供应商（Charles River（CR）和Harlan Laboratories（H））的两种正常血压品系大鼠之间的反应性充血反应是否存在差异，即CR的Sprague-Dawley（SD）大鼠和H的Wistar-Kyoto（WKY）大鼠。将大鼠麻醉，股动脉闭塞5分钟，使用超声血管周围血流探头连续测量股动脉血流。CR的SD大鼠（80±23 mL/min∙s；n = 6）和H的SD大鼠（94±16 mL/min∙s；n = 6）之间，反应性充血反应曲线下的平均面积（持续3分钟）没有差异。如先前报道，H的SD大鼠血压高于CR的SD大鼠。来自两个供应商的WKY大鼠充血明显更大；CR和H的WKY大鼠分别为371±67和281±71 mL/min∙s（n = 5），但同样，不同供应商之间没有差异。不同供应商的WKY大鼠血压相似。这些结果表明，在大鼠中采用改良的FMD方案无法辨别NO生物活性的差异，并且正常血压品系的大鼠尽管血压相似，但反应性充血可能存在很大差异。
