Bhardwaj Archana, Bhardwaj Abhishek, Rao Nageshwar
Department of Conservative Dentistry and Endodontics, Vananchal Dental College and Hospital, Garhwa, Jharkhand, India.
Department of Oral and Maxillofacial Surgery, Vananchal Dental College and Hospital, Garhwa, Jharkhand, India.
J Conserv Dent. 2014 May;17(3):276-9. doi: 10.4103/0972-0707.131799.
The purpose of this study was to evaluate the antifungal action of various concentrations of white mineral trioxide aggregate (MTA) against seven different strains of Candida albicans using the tube dilution test.
Fresh mix of MTA was prepared at concentrations of 100, 50, 25, and 12.5 mg/ml and added to a broth tube containing Sabouraud's liquid medium. A total of 1287 broth tubes were prepared and divided into experimental and control groups. Stock cultures of seven strains of C. albicans were obtained. Fresh inoculate of the microorganism was prepared by growing overnight cultures. Aliquots of the test C. albicans were taken and added to the test tubes. All tubes were incubated at 37°C for 1-, 24-, 72-, and 168-h time periods. At each time period, the presence of C. albicans colonies was assessed.
Differences among the groups were statistically analyzed using Kruskal-Wallis and Mann-Whitney U tests.
Results showed that one strain showed resistance even after 3 days at the lower MTA concentrations of 12.5 and 25 mg/ml. Growth reoccurred with three strains at MTA concentration of 12.5 mg/ml after 7 days. A significant difference was found between strain 3 and other strains at MTA concentrations of 12.5 and 25 mg/ml at the 3-days time period and between tubes containing 12.5 mg/ml and tubes containing higher concentrations of MTA at the 7-days time period.
White MTA in concentrations of 100 and 50 mg/ml is effective in inhibiting the seven tested strains of C. albicans for periods up to 1 week.
本研究旨在通过试管稀释试验评估不同浓度的白色矿物三氧化物凝聚体（MTA）对七种不同白色念珠菌菌株的抗真菌作用。
制备浓度为100、50、25和12.5mg/ml的新鲜MTA混合物，并添加到含有沙氏液体培养基的肉汤管中。共制备了1287个肉汤管，分为实验组和对照组。获得了七种白色念珠菌菌株的储备培养物。通过培养过夜培养物制备微生物的新鲜接种物。取测试白色念珠菌的等分试样并添加到试管中。所有试管在37°C下孵育1、24、72和168小时。在每个时间段，评估白色念珠菌菌落的存在情况。
使用Kruskal-Wallis和Mann-Whitney U检验对组间差异进行统计分析。
结果表明，即使在较低的MTA浓度12.5和25mg/ml下处理3天后，仍有一个菌株显示出抗性。在MTA浓度为12.5mg/ml时，7天后有三种菌株再次生长。在3天时间段，MTA浓度为12.5和25mg/ml时，菌株3与其他菌株之间存在显著差异；在7天时间段，含有12.5mg/ml的试管与含有较高浓度MTA的试管之间存在显著差异。
浓度为100和50mg/ml的白色MTA在长达1周的时间内可有效抑制七种测试的白色念珠菌菌株。
