Mohammadi Zahed, Khademi Abbas Ali, Ezoddini Ardakani Fatemeh
Department of Endodontics, Dental School, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Endodontics, Dental School, Esfahan University of Medical Sciences, Esfahan, Iran.
Iran Endod J. 2006 Winter;1(4):137-40. Epub 2007 Jan 20.
Candida Albicans (CA) is by far the most common yeast of oral infections, including endodontic infections. The aim of this study was to evaluate and compare the antifungal effect of white-colored mineral trioxide aggregate (WMTA) and Portland Cement (PC) using a tube-dilution test.
WMTA and PC were tested freshly mixed and after 24 h. The experiment was performed in 24-well culture plates. Fifty wells were used and divided into four experimental groups (freshly-mixed WMTA freshly-mixed PC, 24 h-set WMTA, and 24 h-set PC) of 10 wells each and control groups of five wells each. Plates of Sabouraud dextrose agar mixed with CA served as positive control and Sabouraud dextrose agar without CA served as negative control. Fresh inoculate of CA was prepared by growing an overnight culture from a stock culture. Aliquots of CA were then taken from the stock culture and plated on the agar compound of the experimental and control groups. All plates were incubated at 37°C for1h, 24 h, and 72 h. Growth of fungi was monitored daily by the presence of turbidity. Kruskal-Wallis test was used for data analysis.
Findings showed that in the freshly mixed as well as 24 h-set WMTA and PC, fungal growth was observed during 1 h incubation; whereas by increasing the incubation time, no fungal growth was observed in 24 h and 72 h.
It was concluded that WMTA and PC (freshly mixed and 24-h set) were effective against CA.
白色念珠菌（CA）是口腔感染（包括牙髓感染）中最常见的酵母菌。本研究的目的是使用试管稀释试验评估和比较白色矿物三氧化物凝聚体（WMTA）和波特兰水泥（PC）的抗真菌效果。
对新鲜混合以及24小时凝固后的WMTA和PC进行测试。实验在24孔培养板中进行。使用50个孔，分为四个实验组（新鲜混合的WMTA、新鲜混合的PC、24小时凝固的WMTA和24小时凝固的PC），每组10个孔，以及每组5个孔的对照组。与CA混合的沙氏葡萄糖琼脂平板用作阳性对照，不含CA的沙氏葡萄糖琼脂平板用作阴性对照。通过从储备培养物中培养过夜培养物来制备CA的新鲜接种物。然后从储备培养物中取出CA的等分试样，接种到实验组和对照组的琼脂复合物上。所有平板在37°C下孵育1小时、24小时和72小时。通过观察浑浊情况每天监测真菌生长。使用Kruskal-Wallis检验进行数据分析。
研究结果表明，在新鲜混合以及24小时凝固的WMTA和PC中，孵育1小时期间观察到真菌生长；而随着孵育时间的增加，在24小时和72小时未观察到真菌生长。
得出的结论是，WMTA和PC（新鲜混合和24小时凝固）对CA有效。
