Kangarlou Ali, Sofiabadi Samira, Asgary Saeed, Mahjour Faranak, Dianat Omid, Yadegari Zahra, Younessian Farnaz
Department of Endodontic, Iranian Center for Endodontic Research, Dental Research Center, Tehran, Iran.
Dent Res J (Isfahan). 2012 May;9(3):256-60.
Fungi may play a key part in periradicular diseases. The aim of this study was to evaluate and compare the antifungal properties of two root-end filling materials, ProRoot Mineral trioxide aggregate (MTA) and MTA-Angelus, against Candida albicans using tube-dilution test.
The antifungal properties of ProRoot MTA and MTA-Angelus against C. albicans was assessed at 1, 24, and 48 hours following administration of two concentrations of the antifungal agents (50 and 100 mg/ml). A total of 50 culture wells were divided into four experimental groups (Freshly mixed MTA, Freshly mixed MTA-Angelus, 24-h set MTA, and 24-h set MTA-Angelus) and two control groups. Each well was prepared for one specific agent with a specific concentration. For the set groups, the mixture was prepared and left for 24 hours. One milliliter of suspension of fungal colonies with concentration of 10(4) CFU/ml was then added to the mixtures in each well. All wells were incubated at 37°C and assessed at 1, 24, and 48 hours. This observation was based on the turbidity of the suspension in the tubes. At each time point, 0.02 ml of each suspension was cultured on a Sabouraud dextrose agar plate to confirm C. albicans growth. The results were analyzed using Kruskal-Wallis test.
Although all fresh and set samples were incapable of killing C. albicans at 1 hour, they demonstrated fungicidal ability on agar plates at 24 and 48-hour time points.
MTA-angelus proved to be an effective antifungal agent compared to ProRoot MTA at concentrations of 50 mg/ml and 100 mg/ml.
真菌可能在根尖周疾病中起关键作用。本研究的目的是使用试管稀释试验评估和比较两种根尖充填材料，即ProRoot三氧化矿物凝聚体（MTA）和MTA-Angelus，对白色念珠菌的抗真菌特性。
在给予两种浓度的抗真菌剂（50和100mg/ml）后1、24和48小时，评估ProRoot MTA和MTA-Angelus对白色念珠菌的抗真菌特性。总共50个培养孔被分为四个实验组（新鲜混合的MTA、新鲜混合的MTA-Angelus、24小时凝固的MTA和24小时凝固的MTA-Angelus）和两个对照组。每个孔针对一种特定浓度的特定试剂进行制备。对于凝固组，制备混合物并放置24小时。然后向每个孔中的混合物中加入1ml浓度为10(4)CFU/ml的真菌菌落悬液。所有孔在37°C下孵育，并在1、24和48小时进行评估。该观察基于试管中悬液的浊度。在每个时间点，将0.02ml的每种悬液接种在沙氏葡萄糖琼脂平板上以确认白色念珠菌的生长。结果采用Kruskal-Wallis检验进行分析。
尽管所有新鲜和凝固的样品在1小时时均无法杀死白色念珠菌，但它们在24和48小时时间点在琼脂平板上显示出杀菌能力。
在50mg/ml和100mg/ml浓度下，与ProRoot MTA相比，MTA-Angelus被证明是一种有效的抗真菌剂。
