Tu Yaosheng, Sun Dongmei, Zeng Xiaohui, Yao Nan, Huang Xuejun, Huang Dane, Chen Yuxing
Traditional Chinese Medicine Pharmacological Research Office, Guangdong Provincial Institute of Traditional Chinese Medicine, Guangzhou, Guangdong 510095, P.R. China.
Exp Ther Med. 2014 Jul;8(1):260-266. doi: 10.3892/etm.2014.1717. Epub 2014 May 16.
It has previously been demonstrated that curcumin possesses a hypocholesterolemic effect and potentiates numerous pharmacological effects of curcumin, however, the mechanisms underlying this hypocholesterolemic effect and the interaction between curcumin and piperine remain to be elucidated. In the present study, male Sprague-Dawley rats were fed on a high-fat diet (HFD) to establish a hyperlipidemia (HLP) model. Co-administration of curcumin plus piperine was found to decrease the levels of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol in the serum and liver, as well as increase the levels of fecal TC, TG and total bile acid, compared with administration of curcumin alone. Curcumin plus piperine also markedly increased the levels of high-density lipoprotein cholesterol. Furthermore, compared with administration of curcumin alone, administration of curcumin plus piperine resulted in a significant upregulation of the activity and gene expression of apolipoprotein AI (ApoAI), lecithin cholesterol acyltransferase (LCAT), cholesterol 7α-hydroxylase (CYP7A1) and low-density lipoprotein receptor (LDLR). In conclusion, these results indicated that co-administration of curcumin plus piperine potentiates the hypocholesterolemic effects of curcumin by increasing the activity and gene expression of ApoAI, CYP7A1, LCAT and LDLR, providing a promising combination for the treatment of HLP.
此前已有研究表明姜黄素具有降胆固醇作用，并增强了姜黄素的多种药理作用，然而，这种降胆固醇作用的潜在机制以及姜黄素与胡椒碱之间的相互作用仍有待阐明。在本研究中，雄性Sprague-Dawley大鼠喂食高脂饮食（HFD）以建立高脂血症（HLP）模型。与单独给予姜黄素相比，发现姜黄素与胡椒碱联合给药可降低血清和肝脏中总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇的水平，并增加粪便中TC、TG和总胆汁酸的水平。姜黄素与胡椒碱联合给药还显著提高了高密度脂蛋白胆固醇的水平。此外，与单独给予姜黄素相比，姜黄素与胡椒碱联合给药导致载脂蛋白AI（ApoAI）、卵磷脂胆固醇酰基转移酶（LCAT）、胆固醇7α-羟化酶（CYP7A1）和低密度脂蛋白受体（LDLR）的活性和基因表达显著上调。总之，这些结果表明，姜黄素与胡椒碱联合给药通过增加ApoAI、CYP7A1、LCAT和LDLR的活性和基因表达来增强姜黄素的降胆固醇作用，为治疗HLP提供了一种有前景的组合。
