Wang Yu-Hong, Yin Ling-Tao, Yang Hui, Li Xin-Liang, Wu Kai-Ge
Hunan Key Laboratory of Chinese Materia Medica Powder and Innovative Drugs Established by Provincial and Ministry, Changsha, Hunan 410007, P.R. China ; Key Laboratory of Internal Medicine of Traditional Chinese Medicine of Ministry of Education, The First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Hunan 410007, P.R. China.
College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, P.R. China.
Exp Ther Med. 2014 Jul;8(1):281-285. doi: 10.3892/etm.2014.1681. Epub 2014 Apr 14.
This study aimed to investigate the hypoglycemic, lipid-lowering and antidepressant effects of Zuogui Jiangtang Jieyu formulation (ZGJTJY) in a model of unpredictable chronic mild stress (UCMS) in rats with diabetes mellitus (DM; the UCMS-DM model). Sixty rats were randomly divided into blank control, vehicle (model plus vehicle), positive control (model plus metformin and fluoxetine), and high, medium and low dose ZGJTJY (model plus high, medium and low doses of ZGJTJY, respectively) groups. Following establishment of DM by a high-fat diet with intraperitoneal injection of streptozotocin (38 mg/kg), the depression model was established by application of UCMS for 28 days. The behavioral scores of the rats were detected in an open field test and Morris water maze test. The levels of blood glucose, glycosylated hemoglobin (HbA1c) and blood lipids were assayed. The total scores of the open field test and the space exploration times (SETs) in the Morris water maze test were significantly lower and the escape latency (EL) times in the Morris water maze test were significantly longer in the vehicle group compared with those in the blank control group. In addition, in the vehicle group, the levels of blood glucose, HbA1c, total cholesterol (TC), triglycerides (TGs) and low-density lipoprotein cholesterol (LDL-C) were significantly higher and the levels of high-density lipoprotein cholesterol (HDL-C) were significantly lower compared with those in the blank control group. The high dose of ZGJTJY decreased the locomotor activity levels in the open field test, the EL times of the model on day 4, the SETs in the Morris water maze test and the HDL-C levels, and reduced the blood glucose, HbA1c, TC, TG and LDL-C levels compared with those in the model group. Thus, ZGJTJY is a potential candidate for the prevention and treatment of the comorbidity of depression with DM.
本研究旨在探讨左归丸降糖解郁方（ZGJTJY）对糖尿病（DM；不可预测慢性轻度应激 - 糖尿病模型，即UCMS - DM模型）大鼠的降血糖、降血脂及抗抑郁作用。将60只大鼠随机分为空白对照组、溶媒组（模型加溶媒）、阳性对照组（模型加二甲双胍和氟西汀）以及高、中、低剂量ZGJTJY组（模型分别加高、中、低剂量ZGJTJY）。通过高脂饮食联合腹腔注射链脲佐菌素（38 mg/kg）建立DM模型后，应用不可预测慢性轻度应激28天建立抑郁模型。在旷场试验和莫里斯水迷宫试验中检测大鼠的行为学评分。测定血糖、糖化血红蛋白（HbA1c）和血脂水平。与空白对照组相比，溶媒组旷场试验的总评分及莫里斯水迷宫试验中的空间探索时间（SETs）显著降低，莫里斯水迷宫试验中的逃避潜伏期（EL）显著延长。此外，与空白对照组相比，溶媒组的血糖、HbA1c、总胆固醇（TC）、甘油三酯（TGs）和低密度脂蛋白胆固醇（LDL - C）水平显著升高而高密度脂蛋白胆固醇（HDL - C）水平显著降低。与模型组相比，高剂量ZGJTJY降低了旷场试验中的运动活动水平、模型第4天的EL时间、莫里斯水迷宫试验中的SETs以及HDL - C水平，并降低了血糖、HbA1c、TC、TG和LDL - C水平。因此，左归丸降糖解郁方是预防和治疗DM合并抑郁症的潜在候选药物。
