菌株的聚合酶链反应核糖体分型、毒素分型及毒素产生的营养方面的比较
Comparison of polymerase chain reaction ribotyping, toxinotyping and nutritional aspects of toxin production of strains.
作者信息
Zhu Shanshan, Zhang Liming, Zhang Chunling, Chen Xianjun, Chen Qi, Li Zhaoyun
机构信息
Department of Clinical Laboratory, Taizhou Central Hospital, Taizhou, Zhejiang 318000, P.R. China.
School of Laboratory and Life Science, Wenzhou Medical University, Wenzhou 325035, P.R. China.
出版信息
Biomed Rep. 2014 Jul;2(4):477-480. doi: 10.3892/br.2014.270. Epub 2014 Apr 25.
Abstract
() is the leading cause of infectious diarrhea in hospitals worldwide. Enterotoxin A (TcdA) and cytotoxin B (TcdB), have been identified as the main virulence factors of . In China, data on polymerase chain reaction (PCR) ribotypes and abilities of hospital-derived isolates to produce TcdA and TcdB are sparse. In this study, we identified 40 isolates from the Taizhou hospital and investigated their PCR ribotypes based on the 16S-23S rRNA gene intergenic spacer region. The ability of different ribotypes to produce TcdA and TcdB was determined by immunochromatography and cytotoxicity assays, respectively. The effects of the nutritional status on the production of these toxins were also investigated.
摘要
（）是全球医院感染性腹泻的主要原因。已确定肠毒素A（TcdA）和细胞毒素B（TcdB）是（）的主要毒力因子。在中国，关于聚合酶链反应（PCR）核糖型以及医院分离的（）菌株产生TcdA和TcdB能力的数据很少。在本研究中，我们从台州医院鉴定出40株（）菌株，并基于16S - 23S rRNA基因间隔区研究了它们的PCR核糖型。分别通过免疫层析和细胞毒性试验确定不同核糖型产生TcdA和TcdB的能力。还研究了营养状况对这些毒素产生的影响。
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