Cao Hung, Yu Fei, Zhao Yu, Zhang Xiaoxiao, Tai Joyce, Lee Juhyun, Darehzereshki Ali, Bersohn Malcolm, Lien Ching-Ling, Chi Neil C, Tai Yu-Chong, Hsiai Tzung K
Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USA.
Integr Biol (Camb). 2014 Aug;6(8):789-95. doi: 10.1039/c4ib00052h.
Understanding the regenerative capacity of small vertebrate models has provided new insights into the plasticity of injured myocardium. Here, we demonstrate the application of flexible microelectrode arrays (MEAs) in elucidating electrophysiological phenotypes of zebrafish and neonatal mouse models of heart regeneration. The 4-electrode MEA membranes were designed to detect electrical signals in the aquatic environment. They were micro-fabricated to adhere to the non-planar body surface of zebrafish and neonatal mice. The acquired signals were processed to display an electrocardiogram (ECG) with high signal-to-noise-ratios, and were validated via the use of conventional micro-needle electrodes. The 4-channel MEA provided signal stability and spatial resolution, revealing the site-specific electrical injury currents such as ST-depression in response to ventricular cryo-injury. Thus, our polymer-based and wearable MEA membranes provided electrophysiological insights into long-term conduction phenotypes for small vertebral models of heart injury and regeneration with a translational implication for monitoring cardiac patients.
对小型脊椎动物模型再生能力的理解为受损心肌的可塑性提供了新的见解。在此，我们展示了柔性微电极阵列（MEA）在阐明斑马鱼和新生小鼠心脏再生模型的电生理表型方面的应用。四电极MEA膜被设计用于检测水生环境中的电信号。它们通过微加工以粘附在斑马鱼和新生小鼠的非平面体表上。采集到的信号经过处理后显示出具有高信噪比的心电图（ECG），并通过使用传统微针电极进行了验证。四通道MEA提供了信号稳定性和空间分辨率，揭示了诸如心室冷冻损伤后ST段压低等位点特异性电损伤电流。因此，我们基于聚合物的可穿戴MEA膜为小型脊椎动物心脏损伤和再生模型的长期传导表型提供了电生理见解，对监测心脏病患者具有转化意义。
