Le Tai, Zhang Jimmy, Xia Xing, Xu Xiaolei, Clark Isaac, Schmiess-Heine Lauren, Nguyen Anh H, Lau Michael P H, Cao Hung
Annu Int Conf IEEE Eng Med Biol Soc. 2020 Jul;2020:2610-2613. doi: 10.1109/EMBC44109.2020.9175576.
The zebrafish model has been demonstrated as an ideal vertebrate model system for a diverse range of biological studies. Along with conventional approaches, monitoring and analysis of zebrafish electrocardiogram (ECG) have been utilized for cardio-physiological screening and elucidation. ECG monitoring has been carried out with fish treated with anesthetic drugs, rendering the short period of time in recording the signals (<5 min). In this work, a prolonged sedation system for continuous ECG monitoring of multiple zebrafish was proposed and developed. We built a circulation system to provide prolonged mild anesthesia which allows more consistent and intrinsic ECG measurement. The use of prolonged anesthesia helped reduce the concentration of the anesthetic drug (MS222 or Tricaine) from 200 mg/L to 100 mg/L and even lower; thus, maintaining the integrity of intrinsic ECG. Moreover, heartrate variation during recording was investigated, showing minute changes (±3.2 beats per minute - BPM). The development of this prolonged ECG monitoring system would open the possibility of long-term monitoring for studies such as drug screening and forward genetic screening.
斑马鱼模型已被证明是用于多种生物学研究的理想脊椎动物模型系统。除了传统方法外，斑马鱼心电图（ECG）的监测和分析已被用于心脏生理筛查和阐释。ECG监测是在使用麻醉药物处理过的鱼身上进行的，记录信号的时间较短（<5分钟）。在这项工作中，提出并开发了一种用于多只斑马鱼连续ECG监测的延长镇静系统。我们构建了一个循环系统，以提供延长的轻度麻醉，从而实现更一致和内在的ECG测量。使用延长麻醉有助于将麻醉药物（MS222或三卡因）的浓度从200 mg/L降低到100 mg/L甚至更低；因此，保持了内在ECG的完整性。此外，还研究了记录过程中的心率变化，显示出微小的变化（每分钟±3.2次心跳 - BPM）。这种延长ECG监测系统的开发将为药物筛选和正向遗传筛选等研究的长期监测开辟可能性。
