INRS-Centre Énergie, Matériaux et Télécommunications, Boulevard Lionel-Boulet, Varennes, Québec J3X 1S2, Canada.
Nanoscale. 2014 Aug 7;6(15):8664-70. doi: 10.1039/c4nr02512a.
We report a new method for the growth of reduced graphene oxide (rGO) on the 316L alloy of stainless steel (SS) and its relevance for biomedical applications. We demonstrate that electrochemical etching increases the concentration of metallic species on the surface and enables the growth of rGO. This result is supported through a combination of Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscopy (SEM), density functional theory (DFT) calculations and static water contact angle measurements. Raman spectroscopy identifies the G and D bands for oxidized species of graphene at 1595 cm(-1) and 1350 cm(-1), respectively, and gives an ID/IG ratio of 1.2, indicating a moderate degree of oxidation. XPS shows -OH and -COOH groups in the rGO stoichiometry and static contact angle measurements confirm the wettability of rGO. SEM and AFM measurements were performed on different substrates before and after coronene treatment to confirm rGO growth. Cell viability studies reveal that these rGO coatings do not have toxic effects on mammalian cells, making this material suitable for biomedical and biotechnological applications.
我们报告了一种在不锈钢（SS）316L 合金上生长还原氧化石墨烯（rGO）的新方法及其在生物医学应用中的相关性。我们证明电化学蚀刻可以增加表面金属物种的浓度，并促进 rGO 的生长。拉曼光谱、X 射线光电子能谱（XPS）、原子力显微镜（AFM）、扫描电子显微镜（SEM）、密度泛函理论（DFT）计算和静态水接触角测量的组合支持了这一结果。拉曼光谱分别在 1595 cm(-1)和 1350 cm(-1)处识别出氧化石墨烯的 G 和 D 带，ID/IG 比为 1.2，表明氧化程度适中。XPS 显示 rGO 化学计量中存在 -OH 和 -COOH 基团，静态接触角测量证实了 rGO 的润湿性。在进行蒄处理前后对不同基底进行 SEM 和 AFM 测量，以确认 rGO 的生长。细胞活力研究表明，这些 rGO 涂层对哺乳动物细胞没有毒性作用，因此这种材料适用于生物医学和生物技术应用。
