Graduate School of Material Science, University of Hyogo , 3-2-1 Kouto, Kamigori, Ako, Hyogo 678-1297, Japan.
Anal Chem. 2014 Jul 15;86(14):6818-22. doi: 10.1021/ac5015996. Epub 2014 Jul 1.
We report a simple device with an array of 10,000 (100 × 100) microwells for producing vertical pairs of cells in individual microwells with a rapid manipulation based on positive dielectrophoresis (p-DEP). The areas encircled with micropoles which fabricated from an electrical insulating photosensitive polymer were used as microwells. The width (14 μm) and depth (25 μm) of the individual microwells restricted the size to two vertically aligned cells. The DEP device for the manipulation of cells consisted of a microfluidic channel with an upper indium tin oxide (ITO) electrode and a lower microwell array electrode fabricated on an ITO substrate. Mouse myeloma cells stained in green were trapped within 1 s in the microwells by p-DEP by applying an alternating current voltage between the upper ITO and the lower microwell array electrode. The cells were retained inside the wells even after switching off the voltage and washing with a fluidic flow. Other myeloma cells stained in blue were then trapped in the microwells occupied by the cells stained in green to form the vertical cell pairing in the microwells. Cells stained in different colors were paired within only 1 min and a pairing efficiency of over 50% was achieved.
我们报告了一种具有 10,000 个（100×100）微井的阵列的简单设备，用于在基于正介电泳（p-DEP）的快速操作下在各个微井中产生垂直对的细胞。用由电绝缘光敏聚合物制成的微电极环绕的区域被用作微井。单个微井的宽度（14μm）和深度（25μm）限制了两个垂直对准的细胞的大小。用于细胞操作的 DEP 装置包括具有上铟锡氧化物（ITO）电极和下微井阵列电极的微流道，该下微井阵列电极在 ITO 基板上制造。通过在上 ITO 和下微井阵列电极之间施加交流电压，用绿色染色的小鼠骨髓瘤细胞在 1s 内被 p-DEP 捕获在微井中。即使在关闭电压并用流体流洗涤后，细胞仍保留在井内。然后，用蓝色染色的其他骨髓瘤细胞被捕获在被绿色染色的细胞占据的微井中，以在微井中形成垂直的细胞对。不同颜色的细胞在 1 分钟内配对，配对效率超过 50%。
