Deelder A M, De Jonge N, Boerman O C, Fillié Y E, Hilberath G W, Rotmans J P, Gerritse M J, Schut D W
Laboratory of Parasitology, State University of Leiden, The Netherlands.
Am J Trop Med Hyg. 1989 Mar;40(3):268-72. doi: 10.4269/ajtmh.1989.40.268.
From a panel of mouse monoclonal antibodies reactive with a repeating epitope of the schistosome circulating anodic antigen, an IgG1 monoclonal antibody was selected. This monoclonal antibody was applied in a sandwich enzyme-linked immunosorbent assay as capture antibody and as alkaline phosphatase labeled conjugate. This assay allowed a sensitive quantitation of circulating anodic antigen in serum samples of infected individuals, detecting less than 1 ng antigen/ml serum. In Schistosoma mansoni infected individuals from Zaire, the level of antigen in serum correlated with fecal egg output. The lower detection level of the immunoassay corresponded to a level of about 10 eggs/gm feces.
从一组与血吸虫循环阳极抗原的重复表位发生反应的小鼠单克隆抗体中，挑选出一种IgG1单克隆抗体。该单克隆抗体被应用于夹心酶联免疫吸附测定中，作为捕获抗体和碱性磷酸酶标记的结合物。该测定法能够灵敏地定量检测感染个体血清样本中的循环阳极抗原，可检测到血清中抗原含量低于1纳克/毫升。在来自扎伊尔的曼氏血吸虫感染个体中，血清中抗原水平与粪便虫卵排出量相关。免疫测定法的较低检测水平对应于约每克粪便10个虫卵的水平。
