Fillié Y E, Van Lieshout L, Kornelis D, Deelder A M
Laboratory for Parasitology, Medical Faculty, University of Leiden, The Netherlands.
Acta Trop. 1994 Sep;57(4):279-87. doi: 10.1016/0001-706x(94)90073-6.
An enzyme-linked immunosorbent assay was developed for combined measurement of schistosome circulating anodic antigen (CAA) and circulating cathodic antigen (CCA). Monoclonal antibodies against CAA and CCA were used as coating and as fluorescein-labeled detecting antibodies in a FITC-anti-FITC system. The lower detection limit of the assay was 1.1 ng antigen (AWA-TCA)/ml. Serum samples of Schistosoma mansoni infected individuals from Zaire (n = 60) and Burundi (n = 60) were tested in this assay and in single-antigen ELISAs. Sensitivities of assaying for CAA, CCA, combined CAA + CCA, and of parallel testing for CAA and for CCA were calculated from titres and antigen concentrations. With serum samples from the heavily infected individuals (Zaire), all assays had a sensitivity of 97% or higher. In contrast, with serum samples from individuals from Burundi (low to moderate infections) it was shown that combined testing resulted in a slightly lower sensitivity than testing for individual antigens. By parallel testing for CAA and CCA, the sensitivity could be increased considerably (to 95%), however.
开发了一种酶联免疫吸附测定法，用于联合检测血吸虫循环阳极抗原（CAA）和循环阴极抗原（CCA）。在异硫氰酸荧光素-抗异硫氰酸荧光素系统中，将针对CAA和CCA的单克隆抗体用作包被抗体和异硫氰酸荧光素标记的检测抗体。该测定法的最低检测限为1.1 ng抗原（AWA-TCA）/ml。用该测定法以及单抗原酶联免疫吸附测定法检测了来自扎伊尔（n = 60）和布隆迪（n = 60）的曼氏血吸虫感染个体的血清样本。根据滴度和抗原浓度计算了检测CAA、CCA、联合检测CAA + CCA以及平行检测CAA和CCA的灵敏度。对于来自重度感染个体（扎伊尔）的血清样本，所有测定法的灵敏度均为97%或更高。相比之下，对于来自布隆迪个体（轻度至中度感染）的血清样本，结果显示联合检测的灵敏度略低于单个抗原检测。然而，通过平行检测CAA和CCA，灵敏度可大幅提高（至95%）。
