de Jonge N, Kremsner P G, Krijger F W, Schommer G, Fillié Y E, Kornelis D, van Zeyl R J, van Dam G J, Feldmeier H, Deelder A M
Laboratory of Parasitology, Medical Faculty, University of Leiden, The Netherlands.
Trans R Soc Trop Med Hyg. 1990 Nov-Dec;84(6):815-8. doi: 10.1016/0035-9203(90)90094-u.
We have developed an enzyme immunoassay (ELISA) for the quantification of the schistosome circulating cathodic antigen (CCA), a glycoprotein associated with the syncitium lining the gut of the parasite. A mouse monoclonal antibody of IgG3 isotype was used as coating (antigen-capture) antibody, while a biotinylated mouse monoclonal IgM was used as second (antigen-detecting) antibody. Streptavidin-alkaline phosphatase was used as enzyme label. The lower detection limit of the assay was 1.0 ng of the trichloroacetic acid soluble fraction of adult worm antigen (AWA-TCA) per ml, which corresponds to approximately 0.2 ng CCA per ml. The ELISA showed a linear range from 1.0 to 62.5 ng AWA-TCA per ml. Serum and urine samples of 16 individuals infected with Schistosoma mansoni (egg counts ranging from 5 to 4820 eggs per gram of faeces) were tested in the assay. Antigen titres ranged from less than 4-8192. This assay represents a considerable advantage in diagnosis of Schistosoma infections as it allows the detection and quantification of CCA in serum and urine in even lightly infected individuals.
我们开发了一种酶免疫测定法（ELISA），用于定量检测血吸虫循环阴极抗原（CCA），这是一种与寄生虫肠道内衬合胞体相关的糖蛋白。使用IgG3同种型的小鼠单克隆抗体作为包被（抗原捕获）抗体，而生物素化的小鼠单克隆IgM用作第二（抗原检测）抗体。链霉亲和素-碱性磷酸酶用作酶标记。该测定法的最低检测限为每毫升1.0纳克成虫抗原的三氯乙酸可溶部分（AWA-TCA），这相当于每毫升约0.2纳克CCA。ELISA显示每毫升AWA-TCA的线性范围为1.0至62.5纳克。对16名感染曼氏血吸虫的个体（每克粪便中的虫卵计数范围为5至4820个虫卵）的血清和尿液样本进行了该测定法检测。抗原滴度范围为小于4至8192。该测定法在血吸虫感染诊断中具有相当大的优势，因为它能够检测和定量即使是轻度感染个体血清和尿液中的CCA。
