利用间接血凝试验对曼氏血吸虫病患者循环抗原进行定量测定。
Quantitative determination of circulating antigens in human schistosomiasis mansoni using an indirect hemagglutination assay.
作者信息
Deelder A M, De Jonge N, Fillié Y E, Kornelis D, Helaha D, Qian Z L, De Caluwé P, Polderman A M
机构信息
Laboratory of Parasitology, Medical Faculty, State University of Leiden, The Netherlands.
出版信息
Am J Trop Med Hyg. 1989 Jan;40(1):50-4. doi: 10.4269/ajtmh.1989.40.50.
In serum and urine specimens collected from a group of Schistosoma mansoni infected individuals from Makundju, Zaire, the schistosome circulating anodic antigen (CAA) and the circulating cathodic antigen (CCA) were quantitatively determined using an indirect hemagglutination reaction with sheep erythrocytes sensitized with mouse IgM monoclonal antibodies directed against these circulating antigens. Levels of CAA in serum (up to 5 ng/ml) and CCA in serum and urine (up to 50 ng/ml) were strongly correlated with egg excretion and with each other. No correlation was found between egg excretion and antibody levels against the circulating antigens. Antigen was detectable only in patients excreting greater than 500 eggs per gram of feces.
在从扎伊尔马昆朱的一组曼氏血吸虫感染个体采集的血清和尿液标本中，使用针对这些循环抗原的小鼠IgM单克隆抗体致敏的绵羊红细胞间接血凝反应，对血吸虫循环阳极抗原（CAA）和循环阴极抗原（CCA）进行了定量测定。血清中CAA的水平（高达5 ng/ml）以及血清和尿液中CCA的水平（高达50 ng/ml）与虫卵排泄量密切相关，且两者之间也密切相关。未发现虫卵排泄量与针对循环抗原的抗体水平之间存在相关性。仅在每克粪便中排出超过500个虫卵的患者中可检测到抗原。
Zotero 插件