Lin Shasha, Lu Guozhong, Liu Shanshan, Bai Shumeng, Liu Xi, Lu Qiang, Zuo Baoqi, Kaplan David L, Zhu Hesun
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, People's Republic of China.
Department of Burns and Plastic Surgery, The Third Affiliated Hospital of Nantong University, Wuxi 214041, People's Republic of China.
J Mater Chem B. 2014 May 7;2(17):2622-2633. doi: 10.1039/C4TB00019F.
Silk-based porous scaffolds have been used extensively in tissue engineering because of their excellent biocompatibility, tunable biodegradability and robust mechanical properties. Although many silk-based scaffolds have been prepared through freeze-drying, a challenge remains to effectively control porous structures during this process. In the present study silk fibroin with different nanostructures were self-assembled in aqueous solution by repeated drying-dissolving process and then used to improve porous structure formation in lyophilization process. Viscosity, secondary structures and water interactions were also studied to exclude their influence on the formation and control of porous structures. Following nanofiber formation in aqueous solution, silk scaffolds with improved porous structure were directly formed after lyophilization and then stabilized with water or methanol annealing treatments. Compared to silk scaffolds derived from fresh solution, the nanofibrous scaffolds showed significantly better cell compatibility . Therefore, this nanoscale control of silk offers feasible way to regulate the matrix features including porous structure and nanostructure, which are important in regulating cell and tissue outcomes in tissue engineering and regeneration, and then achieve silk-based scaffolds with improved properties.
基于丝绸的多孔支架因其优异的生物相容性、可调节的生物降解性和强大的机械性能而在组织工程中得到广泛应用。尽管许多基于丝绸的支架是通过冷冻干燥制备的，但在此过程中有效控制多孔结构仍然是一个挑战。在本研究中，具有不同纳米结构的丝素蛋白通过反复干燥-溶解过程在水溶液中自组装，然后用于改善冻干过程中多孔结构的形成。还研究了粘度、二级结构和水相互作用，以排除它们对多孔结构形成和控制的影响。在水溶液中形成纳米纤维后，经冷冻干燥直接形成具有改善的多孔结构的丝绸支架，然后通过水或甲醇退火处理使其稳定。与源自新鲜溶液的丝绸支架相比，纳米纤维支架表现出明显更好的细胞相容性。因此，这种对丝绸的纳米级控制提供了一种可行的方法来调节包括多孔结构和纳米结构在内的基质特征，这些特征在组织工程和再生中调节细胞和组织结果方面很重要，进而获得具有改进性能的基于丝绸的支架。
