Naval Surface Warfare Center, Dahlgren Division, CBR Concepts and Experimentation Branch, 4045 Higley Road Suite 345, Dahlgren VA 22448-5162, USA.
AMB Express. 2014 May 1;4:21. doi: 10.1186/s13568-014-0021-3. eCollection 2014.
Response surface methodology using a face-centered cube design was used to describe and predict spore inactivation of Bacillus anthracis ∆Sterne and Bacillus thuringiensis Al Hakam spores after exposure of six spore-contaminated materials to hot, humid air. For each strain/material pair, an attempt was made to fit a first or second order model. All three independent predictor variables (temperature, relative humidity, and time) were significant in the models except that time was not significant for B. thuringiensis Al Hakam on nylon. Modeling was unsuccessful for wiring insulation and wet spores because there was complete spore inactivation in the majority of the experimental space. In cases where a predictive equation could be fit, response surface plots with time set to four days were generated. The survival of highly purified Bacillus spores can be predicted for most materials tested when given the settings for temperature, relative humidity, and time. These predictions were cross-checked with spore inactivation measurements.
采用基于中心点立方设计的响应面方法，描述并预测了炭疽芽孢杆菌 ∆Sterne 孢子和苏云金芽孢杆菌 Al Hakam 孢子在暴露于六种污染有孢子的材料于湿热空气中后的失活动力学。对于每一对菌株/材料，都尝试拟合一阶或二阶模型。除了在尼龙上的苏云金芽孢杆菌 Al Hakam 外，所有三个独立预测变量（温度、相对湿度和时间）在模型中均显著，时间对其不显著。由于在大多数实验空间中完全失活，因此对电线绝缘和湿孢子的建模不成功。在可以拟合预测方程的情况下，生成了时间设置为四天的响应面图。在给定温度、相对湿度和时间的情况下，大多数测试材料中的高纯度芽孢杆菌孢子的存活情况都可以进行预测。这些预测与孢子失活测量结果进行了交叉检查。
