Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, PR China.
School of Chemistry and Australian Centre for NanoMedicine, The University of New South Wales, Sydney, NSW 2052, Australia.
Biosens Bioelectron. 2014 Nov 15;61:491-9. doi: 10.1016/j.bios.2014.05.039. Epub 2014 Jun 3.
The loop-mediated isothermal amplification (LAMP) technique has the potential to revolutionize molecular biology because it allows DNA amplification under isothermal conditions and is highly compatible with point-of-care analysis. To achieve efficient genetic analysis of samples, the method of real-time or endpoint determination selected to monitor the biochemical reaction is of great importance. In this paper we briefly review progress in the development of monitoring methods for LAMP.
环介导等温扩增（LAMP）技术有可能引发分子生物学的革命，因为它可以在等温条件下进行 DNA 扩增，并且与即时检测分析高度兼容。为了实现对样本的高效基因分析，选择用于监测生化反应的实时或终点测定方法非常重要。本文简要综述了 LAMP 监测方法的研究进展。
