• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

抗白细胞介素 39（白细胞介素 23p19/Ebi3）多克隆抗体可改善狼疮样小鼠的自身免疫症状。

Anti‑IL‑39 (IL‑23p19/Ebi3) polyclonal antibodies ameliorate autoimmune symptoms in lupus‑like mice.

机构信息

Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.

College of Pharmacy, Henan University, Kaifeng, Henan 475004, P.R. China.

出版信息

Mol Med Rep. 2018 Jan;17(1):1660-1666. doi: 10.3892/mmr.2017.8048. Epub 2017 Nov 14.

DOI:10.3892/mmr.2017.8048
PMID:29138852
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5780108/
Abstract

The interleukin (IL)‑12 family cytokines have been examined as therapeutic targets in the treatment of several autoimmune diseases. Our previous study showed that a novel IL‑12 family cytokine, IL‑39 (IL‑23p19/Ebi3) mediates inflammation in lupus‑like mice. In the present study, the effect of anti‑mouse IL‑39 polyclonal antibodies on autoimmune symptoms in lupus‑like mice was investigated. Rabbit anti‑mouse IL‑39 polyclonal antibodies were produced by immunization with recombinant mouse IL‑39, and purified using protein A chromatography. These antibodies were subsequently used to treat lupus‑like mice. Flow cytometry, captured images, ELISA and H&E staining were used to determine the effect of anti‑IL‑39 polyclonal antibodies on inflammatory cells, autoantibody titers, proteinuria, infiltrating inflammatory cells and the structure of the glomerular region. The anti‑IL‑39 polyclonal antibodies effectively reduced the numbers of inflammatory cells, splenomegaly, autoantibody titers, proteinuria, infiltrating inflammatory cells, and restored the structure of the glomerular region in MRL/lpr mice. Taken together, these results suggested that anti‑IL‑39 polyclonal antibodies ameliorated autoimmune symptoms in lupus‑like mice. Therefore, IL‑39 may be used as a possible target for the treatment of systemic lupus erythematosus.

摘要

白细胞介素 (IL)‑12 家族细胞因子已被研究作为几种自身免疫性疾病治疗的靶点。我们之前的研究表明，一种新型的白细胞介素 12 家族细胞因子，IL‑39 (IL‑23p19/Ebi3)，在狼疮样小鼠中介导炎症。在本研究中，研究了抗小鼠 IL‑39 多克隆抗体对狼疮样小鼠自身免疫症状的影响。通过用重组小鼠 IL‑39 免疫兔产生兔抗小鼠 IL‑39 多克隆抗体，并使用蛋白 A 层析法纯化。随后将这些抗体用于治疗狼疮样小鼠。流式细胞术、捕获图像、ELISA 和 H&E 染色用于确定抗 IL‑39 多克隆抗体对炎症细胞、自身抗体滴度、蛋白尿、浸润性炎症细胞和肾小球区域结构的影响。抗 IL‑39 多克隆抗体可有效减少炎症细胞、脾肿大、自身抗体滴度、蛋白尿、浸润性炎症细胞的数量，并恢复 MRL/lpr 小鼠肾小球区域的结构。综上所述，这些结果表明抗 IL‑39 多克隆抗体改善了狼疮样小鼠的自身免疫症状。因此，IL‑39 可能被用作治疗系统性红斑狼疮的潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/c6271aaf8370/MMR-17-01-1660-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/e61021996294/MMR-17-01-1660-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/c967e1075b08/MMR-17-01-1660-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/1fa4f3bf6a76/MMR-17-01-1660-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/275bb06fcfbc/MMR-17-01-1660-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/d8e73477c94a/MMR-17-01-1660-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/c6271aaf8370/MMR-17-01-1660-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/e61021996294/MMR-17-01-1660-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/c967e1075b08/MMR-17-01-1660-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/1fa4f3bf6a76/MMR-17-01-1660-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/275bb06fcfbc/MMR-17-01-1660-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/d8e73477c94a/MMR-17-01-1660-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4dc7/5780108/c6271aaf8370/MMR-17-01-1660-g05.jpg

相似文献

1
Anti‑IL‑39 (IL‑23p19/Ebi3) polyclonal antibodies ameliorate autoimmune symptoms in lupus‑like mice.抗白细胞介素 39（白细胞介素 23p19/Ebi3）多克隆抗体可改善狼疮样小鼠的自身免疫症状。
Mol Med Rep. 2018 Jan;17(1):1660-1666. doi: 10.3892/mmr.2017.8048. Epub 2017 Nov 14.
2
Aconitine: A potential novel treatment for systemic lupus erythematosus.乌头碱：一种治疗系统性红斑狼疮的潜在新疗法。
J Pharmacol Sci. 2017 Mar;133(3):115-121. doi: 10.1016/j.jphs.2017.01.007. Epub 2017 Feb 24.
3
IL-12-encoding plasmid has a beneficial effect on spontaneous autoimmune disease in MRL/MP-lpr/lpr mice.编码白细胞介素-12的质粒对MRL/MP-lpr/lpr小鼠的自发性自身免疫疾病有有益作用。
Cytokine. 2000 Jul;12(7):1035-41. doi: 10.1006/cyto.1999.0662.
4
Amelioration of systemic lupus erythematosus by Withangulatin A in MRL/lpr mice.白杨素 A 改善 MRL/lpr 小鼠的系统性红斑狼疮。
J Cell Biochem. 2011 Sep;112(9):2376-82. doi: 10.1002/jcb.23160.
5
Prednisone treatment inhibits the differentiation of B lymphocytes into plasma cells in MRL/MpSlac-lpr mice.泼尼松治疗可抑制MRL/MpSlac-lpr小鼠中B淋巴细胞向浆细胞的分化。
Acta Pharmacol Sin. 2015 Nov;36(11):1367-76. doi: 10.1038/aps.2015.76. Epub 2015 Oct 12.
6
Histone deacetylase inhibitors modulate renal disease in the MRL-lpr/lpr mouse.组蛋白去乙酰化酶抑制剂可调节MRL-lpr/lpr小鼠的肾脏疾病。
J Clin Invest. 2003 Feb;111(4):539-52. doi: 10.1172/JCI16153.
7
Deficiency in EBV-induced gene 3 (EBI3) in MRL/lpr mice results in pathological alteration of autoimmune glomerulonephritis and sialadenitis.MRL/lpr小鼠中EB病毒诱导基因3（EBI3）的缺陷会导致自身免疫性肾小球肾炎和涎腺炎的病理改变。
Mod Rheumatol. 2009;19(1):33-41. doi: 10.1007/s10165-008-0117-1. Epub 2008 Sep 10.
8
Lack of evidence for expression and function of IL-39 in human immune cells.缺乏人免疫细胞中 IL-39 表达和功能的证据。
PLoS One. 2020 Dec 1;15(12):e0242329. doi: 10.1371/journal.pone.0242329. eCollection 2020.
9
Activated protein C attenuates systemic lupus erythematosus and lupus nephritis in MRL-Fas(lpr) mice.活化蛋白 C 可减轻 MRL-Fas(lpr) 小鼠的系统性红斑狼疮和狼疮肾炎。
J Immunol. 2011 Sep 15;187(6):3413-21. doi: 10.4049/jimmunol.1101125. Epub 2011 Aug 17.
10
A novel IL-23p19/Ebi3 (IL-39) cytokine mediates inflammation in Lupus-like mice.一种新型白细胞介素-23 p19/Ebi3（白细胞介素-39）细胞因子介导狼疮样小鼠的炎症反应。
Eur J Immunol. 2016 Jun;46(6):1343-50. doi: 10.1002/eji.201546095. Epub 2016 Apr 13.

引用本文的文献

1
IL-39 and IL-35 gingival crevicular fluid levels in diabetic patients with generalized periodontitis.白细胞介素 39 和白细胞介素 35 在患有广泛性牙周炎的糖尿病患者的龈沟液中的水平。
Clin Oral Investig. 2024 Jan 30;28(2):124. doi: 10.1007/s00784-023-05484-3.
2
Evaluation of IL-23p19/Ebi3 (IL-39) gingival crevicular fluid levels in periodontal health, gingivitis, and periodontitis.评估牙周健康、牙龈炎和牙周炎患者龈沟液中白细胞介素-23p19/Ebi3（白细胞介素-39）水平。
Clin Oral Investig. 2022 Dec;26(12):7209-7218. doi: 10.1007/s00784-022-04681-w. Epub 2022 Aug 20.
3
IL-39 promotes chronic graft-versus-host disease by increasing T and B Cell pathogenicity.

本文引用的文献

1
Interleukin (IL)-39 [IL-23p19/Epstein-Barr virus-induced 3 (Ebi3)] induces differentiation/expansion of neutrophils in lupus-prone mice.白细胞介素（IL）-39 [IL-23p19/EB病毒诱导基因3（Ebi3）]可诱导狼疮易感小鼠中性粒细胞的分化/扩增。
Clin Exp Immunol. 2016 Nov;186(2):144-156. doi: 10.1111/cei.12840. Epub 2016 Aug 12.
2
A novel IL-23p19/Ebi3 (IL-39) cytokine mediates inflammation in Lupus-like mice.一种新型白细胞介素-23 p19/Ebi3（白细胞介素-39）细胞因子介导狼疮样小鼠的炎症反应。
Eur J Immunol. 2016 Jun;46(6):1343-50. doi: 10.1002/eji.201546095. Epub 2016 Apr 13.
3
Pre-existing CD19-independent GL7(-) Breg cells are expanded during inflammation and in mice with lupus-like disease.
白细胞介素-39通过增加T细胞和B细胞的致病性来促进慢性移植物抗宿主病。
Exp Hematol Oncol. 2022 Jun 2;11(1):34. doi: 10.1186/s40164-022-00286-x.
4
Multifaceted Analysis of IL-23A- and/or EBI3-Including Cytokines Produced by Psoriatic Keratinocytes.银屑病角质形成细胞产生的细胞因子的 IL-23A 和/或 EBI3 分析。
Int J Mol Sci. 2021 Nov 23;22(23):12659. doi: 10.3390/ijms222312659.
5
Lack of evidence for expression and function of IL-39 in human immune cells.缺乏人免疫细胞中 IL-39 表达和功能的证据。
PLoS One. 2020 Dec 1;15(12):e0242329. doi: 10.1371/journal.pone.0242329. eCollection 2020.
6
Gm614 Protects Germinal Center B Cells From Death by Suppressing Caspase-1 Transcription in Lupus-Prone Mice.Gm614 通过抑制狼疮易感小鼠中的胱天蛋白酶-1 转录来保护生发中心 B 细胞免于死亡。
Front Immunol. 2020 Oct 21;11:585726. doi: 10.3389/fimmu.2020.585726. eCollection 2020.
7
T2 Peptide Represents a Major Autoantigen Epitope in Experimental Autoimmune Prostatitis.T2 肽是实验性自身免疫性前列腺炎的主要自身抗原表位。
Inflammation. 2021 Feb;44(1):243-248. doi: 10.1007/s10753-020-01326-6. Epub 2020 Aug 22.
8
Inhibition of IRF4 in dendritic cells by PRR-independent and -dependent signals inhibit Th2 and promote Th17 responses.通过非依赖于 PRR 和依赖于 PRR 的信号抑制树突状细胞中的 IRF4 可抑制 Th2 并促进 Th17 反应。
Elife. 2020 Feb 4;9:e49416. doi: 10.7554/eLife.49416.
9
IL-27, IL-30, and IL-35: A Cytokine Triumvirate in Cancer.白细胞介素-27、白细胞介素-30和白细胞介素-35：癌症中的细胞因子三联体
Front Oncol. 2019 Oct 1;9:969. doi: 10.3389/fonc.2019.00969. eCollection 2019.
10
IL-39 acts as a friend to pancreatic cancer.白细胞介素 39 是胰腺癌的“朋友”。
Med Oncol. 2018 Dec 1;36(1):12. doi: 10.1007/s12032-018-1236-y.
预先存在的不依赖CD19的GL7(-)调节性B细胞在炎症期间以及狼疮样疾病小鼠中会扩增。
Mol Immunol. 2016 Mar;71:54-63. doi: 10.1016/j.molimm.2016.01.011. Epub 2016 Feb 4.
4
IL-12 and IL-23 cytokines: from discovery to targeted therapies for immune-mediated inflammatory diseases.IL-12 和 IL-23 细胞因子：从发现到针对免疫介导的炎症性疾病的靶向治疗。
Nat Med. 2015 Jul;21(7):719-29. doi: 10.1038/nm.3895. Epub 2015 Jun 29.
5
Ligation of metabotropic glutamate receptor 3 (Grm3) ameliorates lupus-like disease by reducing B cells.代谢型谷氨酸受体3（Grm3）的结扎通过减少B细胞改善狼疮样疾病。
Clin Immunol. 2015 Oct;160(2):142-54. doi: 10.1016/j.clim.2015.05.016. Epub 2015 Jun 9.
6
The use of systematic reviews and reporting guidelines to advance the implementation of the 3Rs.利用系统评价和报告指南推进3R原则的实施。
J Am Assoc Lab Anim Sci. 2015 Mar;54(2):153-62.
7
Interleukin 12 (IL-12) family cytokines: Role in immune pathogenesis and treatment of CNS autoimmune disease.白细胞介素12（IL-12）家族细胞因子：在中枢神经系统自身免疫性疾病免疫发病机制及治疗中的作用
Cytokine. 2015 Oct;75(2):249-55. doi: 10.1016/j.cyto.2015.01.030. Epub 2015 Mar 18.
8
Critical role for thymic CD19+CD5+CD1dhiIL-10+ regulatory B cells in immune homeostasis.胸腺CD19+CD5+CD1dhiIL-10+调节性B细胞在免疫稳态中起关键作用。
J Leukoc Biol. 2015 Mar;97(3):547-56. doi: 10.1189/jlb.3A0414-213RR. Epub 2014 Dec 16.
9
Production of IgG autoantibody requires expression of activation-induced deaminase in early-developing B cells in a mouse model of SLE.在系统性红斑狼疮小鼠模型中，IgG自身抗体的产生需要早期发育的B细胞中激活诱导的脱氨酶的表达。
Eur J Immunol. 2014 Oct;44(10):3093-108. doi: 10.1002/eji.201344282. Epub 2014 Aug 19.
10
Neutrophils contribute to excess serum BAFF levels and promote CD4+ T cell and B cell responses in lupus-prone mice.中性粒细胞导致血清中B淋巴细胞刺激因子（BAFF）水平过高，并促进狼疮易感小鼠的CD4+ T细胞和B细胞反应。
PLoS One. 2014 Jul 10;9(7):e102284. doi: 10.1371/journal.pone.0102284. eCollection 2014.