Issekutz T B, Stoltz J M
Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia, Canada.
Cell Immunol. 1989 Apr 15;120(1):165-73. doi: 10.1016/0008-8749(89)90184-6.
Since several studies have demonstrated that lipopolysaccharide (LPS), tumor necrosis factor (TNF), and interleukin-1 (IL-1) enhanced lymphocyte binding to endothelial cells in vitro, we examined the effects of these agents on lymphocyte migration in vivo. Small peritoneal exudate lymphocytes (sPEL), which perferentially migrate into inflammatory sites, were radiolabeled with 111In and injected iv into rats. The id injection of LPS was a strong stimulus for the migration of these cells into the skin. TNF alpha was also a good stimulator of lymphocyte migration, while TNF beta and IL-1 alpha were weak or nearly inactive. Kinetic analysis demonstrated that migration to TNF was rapid, with a peak at 6 hr, followed by a steady decline, while migration to LPS was sustained for 24 hr. TNF alpha, TNF beta, and IL-1 alpha, when combined with interferon-gamma (IFN-gamma) or IFN-alpha/beta produced striking synergistic increases in lymphocyte migration. Combinations of the TNFs and IL-1 had less than additive effects, as did combinations of the IFNs. Qualitatively similar migration responses were found when spleen T cells instead of sPEL were studied.
由于多项研究已证明脂多糖（LPS）、肿瘤坏死因子（TNF）和白细胞介素-1（IL-1）在体外可增强淋巴细胞与内皮细胞的结合，我们研究了这些因子对体内淋巴细胞迁移的影响。优先迁移至炎症部位的小腹腔渗出淋巴细胞（sPEL）用铟-111进行放射性标记，然后静脉注射到大鼠体内。皮下注射LPS是这些细胞迁移至皮肤的强烈刺激因素。肿瘤坏死因子α（TNFα）也是淋巴细胞迁移的良好刺激物，而肿瘤坏死因子β（TNFβ）和白细胞介素-1α（IL-1α）则作用较弱或几乎无活性。动力学分析表明，向TNF的迁移迅速，在6小时达到峰值，随后稳定下降，而向LPS的迁移可持续24小时。TNFα、TNFβ和IL-1α与干扰素-γ（IFN-γ）或干扰素-α/β联合使用时，可显著协同增加淋巴细胞迁移。TNF与IL-1的组合以及IFN的组合，其作用均小于相加效应。当研究脾T细胞而非sPEL时，发现了定性相似的迁移反应。
