Sadeghi Susan, Jahani Moslem, Belador Foroogh
Department of Chemistry, Faculty of Science, University of Birjand, Birjand, Iran.
Department of Chemistry, Faculty of Science, University of Birjand, Birjand, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Apr 15;159:83-9. doi: 10.1016/j.saa.2016.01.043. Epub 2016 Jan 25.
The Mn doped ZnS quantum dots (Mn:ZnS QDs) capped with the florfenicol molecularly imprinted polymer (Mn:ZnS QDs@MIP) were prepared via the sol-gel surface imprinting approach using 3-aminopropyltriethoxysilane (APTES) as the functional monomer and tetraethoxysilane (TEOS) as the cross-linker for the optosensing of the florfenicol. Transmission electron microscopy (TEM), X-ray diffractometer, IR spectroscopy, UV-Vis absorption spectrophotometry, and spectrofluorometry were used to elucidate the formation, morphology, and identification of the products. To illustrate the usefulness of the new imprinted material, the non-imprinted coated Mn:ZnS QDs (Mn:ZnS QDs@NIP) were synthesized without the presence of the florfenicol. It was revealed that the fluorescence (FL) intensity of the Mn:ZnS QDs@MIP increased with increasing the FF concentration. Under the optimal conditions, changes in the FL intensity in the presence of the target molecule showed a linear response in the concentration range of 30-700 μmol L(-1) with a detection limit of 24 μmol L(-1). The developed method was finally applied successfully to the determination of FF in different meat samples with satisfactory recoveries.
以氟苯尼考分子印迹聚合物为封端剂的锰掺杂硫化锌量子点（Mn:ZnS QDs@MIP），采用溶胶 - 凝胶表面印迹法，以3 - 氨丙基三乙氧基硅烷（APTES）为功能单体，正硅酸乙酯（TEOS）为交联剂，用于氟苯尼考的光传感检测。通过透射电子显微镜（TEM）、X射线衍射仪、红外光谱、紫外 - 可见吸收分光光度法和荧光光谱法对产物的形成、形态和结构进行了表征。为了说明新型印迹材料的实用性，在不存在氟苯尼考的情况下合成了非印迹包覆的Mn:ZnS QDs（Mn:ZnS QDs@NIP）。结果表明，Mn:ZnS QDs@MIP的荧光（FL）强度随氟苯尼考（FF）浓度的增加而增强。在最佳条件下，目标分子存在时荧光强度的变化在30 - 700 μmol L(-1)浓度范围内呈线性响应，检测限为24 μmol L(-1)。该方法最终成功应用于不同肉类样品中氟苯尼考的测定，回收率令人满意。
