Gibbons R A, Martinez O M, Lim R C, Horn J K, Garovoy M R
Immunogenetics and Transplantation Laboratory, Department of Surgery, University of California, San Francisco 94143.
Clin Exp Immunol. 1989 Mar;75(3):371-5.
Monocytes that bear HLA Class II antigens, such as HLA-DR, HLA-DQ, or HLA-DP, are obligatory for many cell-mediated immunological processes. Patients with thermal injury suffer from hypoimmunity and are at risk for developing life-threatening septic episodes. To determine whether an alteration in expression of HLA Class II antigens is involved in the defect, monocytes from the peripheral blood of burn patients and controls were double-stained with anti-Leu-M3 and either anti-HLA-DR, HLA-DQ, or HLA-DP monoclonal antibodies. As analysed by flow cytometry the percentage of Leu-M3+ monocytes from the peripheral blood from patients and controls was the same. The percentage of Leu-M3+ monocytes bearing the HLA Class II antigens and the density of antigen on the monocytes, however, was significantly reduced post-burn compared with controls. In nearly all cases these changes were detected as early as 24 h post-burn before any drug therapy was implemented. In-vivo re-expression of normal levels of HLA Class II coincided with patient recovery. In-vitro exposure of post-burn Leu-M3+ cells to IFN-gamma for 72 h restored HLA Class II expression to control levels. It is possible that the reductions in HLA Class II expression may be involved in the general immunosuppression that follows thermal injury.
携带HLA-II类抗原（如HLA-DR、HLA-DQ或HLA-DP）的单核细胞对许多细胞介导的免疫过程至关重要。热损伤患者存在免疫功能低下，有发生危及生命的败血症的风险。为了确定HLA-II类抗原表达的改变是否与这种缺陷有关，对烧伤患者和对照组外周血中的单核细胞用抗-Leu-M3和抗-HLA-DR、HLA-DQ或HLA-DP单克隆抗体进行双重染色。通过流式细胞术分析，患者和对照组外周血中Leu-M3 +单核细胞的百分比相同。然而，与对照组相比，烧伤后携带HLA-II类抗原的Leu-M3 +单核细胞百分比以及单核细胞上抗原的密度显著降低。在几乎所有情况下，这些变化早在烧伤后24小时就被检测到，此时尚未实施任何药物治疗。HLA-II类抗原正常水平的体内重新表达与患者康复同步。烧伤后Leu-M3 +细胞在体外暴露于γ干扰素72小时可使HLA-II类抗原表达恢复到对照水平。HLA-II类抗原表达的降低可能与热损伤后的全身免疫抑制有关。
