Stein M D, Gamble D N, Klimpel K D, Herndon D N, Klimpel G R
Cell Immunol. 1984 Jul;86(2):551-6. doi: 10.1016/0008-8749(84)90412-x.
Peripheral blood mononuclear lymphocytes (PBL) from thermal injury patients were examined for their ability to mediate spontaneous (NK) cytotoxic activity against K562 tumor cells. NK cell numbers were quantitated in burn patients using the fluorescein isothiocyanate-conjugated monoclonal antibody Leu-7 and an Ortho cell sorter. NK activity of burn patients was only 30% of control levels. Thus, at a 50:1 effector:target cell ratio, burn patients' PBL gave a mean of 18% killing as compared to 60% killing mediated by control PBL (14 individuals). Pretreatment of PBL with interferon-gamma or interferon-alpha enhanced NK activity of normal PBL but had no effect on the NK activity of PBL from burn patients. The number of cells bearing antigen recognized by Leu-7 was variable in burn patients, with some patients having decreased (less than 5%), normal (8-10%), or elevated (20-40%) numbers of Leu-7 bearing PBL. No differences were observed in the total numbers of PBL in burn patients when compared to control individuals. These results indicate that NK activity does not correlate with the percentage of Leu-7 bearing cells present in PBL and that the inability of thermal injury patients to mediate normal NK function may contribute to their susceptibilities to viral infections.
检测了热损伤患者外周血单个核淋巴细胞（PBL）对K562肿瘤细胞介导自发（NK）细胞毒性活性的能力。使用异硫氰酸荧光素偶联的单克隆抗体Leu-7和Ortho细胞分选仪对烧伤患者的NK细胞数量进行定量。烧伤患者的NK活性仅为对照水平的30%。因此，在效应细胞与靶细胞比例为50:1时，烧伤患者的PBL平均杀伤率为18%，而对照PBL（14名个体）介导的杀伤率为60%。用γ干扰素或α干扰素预处理PBL可增强正常PBL的NK活性，但对烧伤患者PBL的NK活性没有影响。烧伤患者中被Leu-7识别的携带抗原的细胞数量各不相同，一些患者携带Leu-7的PBL数量减少（低于5%）、正常（8 - 10%）或升高（20 - 40%）。与对照个体相比，烧伤患者的PBL总数没有差异。这些结果表明，NK活性与PBL中携带Leu-7的细胞百分比无关，热损伤患者无法介导正常的NK功能可能导致他们易受病毒感染。
