Garza-Ramos Ulises, Barrios Humberto, Reyna-Flores Fernando, Sánchez-Pérez Alejandro, Tamayo-Legorreta Elsa, Ibarra-Pacheco Alvaro, Salazar-Salinas Juana, Núñez-Ceballos Ricardo, Silva-Sanchez Jesus
Departamento de Diagnóstico Epidemiológico, Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
Hospital Regional 1(o) Octubre ISSSTE, México, DF, Mexico.
Diagn Microbiol Infect Dis. 2014 Aug;79(4):483-5. doi: 10.1016/j.diagmicrobio.2014.05.010. Epub 2014 May 20.
The KPC-producing Klebsiella pneumoniae sequence type 258 (ST258) is an important pathogen widely spread in nosocomial infections. In this study, we identified the KPC-2-producing K. pneumoniae clinical isolates of 2 unrelated outbreaks that corresponded to pandemic strain ST258. The isolates showed high resistance to cephalosporins, carbapenems, quinolones, and colistin. The KPC-2-producing K. pneumoniae isolates were compared to the previously studied KPC-3-producing K. pneumoniae isolates from an outbreak in Mexico; they showed an unrelated pulsed-field gel electrophoresis fingerprinting pattern and a different plasmid profile. The KPC-2 carbapenemase gene was identified in two 230- and 270-kb non-conjugative plasmids; however, 1 isolate transferred the KPC-2 gene onto an 80-kb plasmid. These findings endorse the need of carrying out a continuous molecular epidemiological surveillance of carbapenem-resistant isolates in hospitals in Mexico.
产KPC的肺炎克雷伯菌序列型258（ST258）是一种在医院感染中广泛传播的重要病原体。在本研究中，我们鉴定了2起不相关暴发中产生KPC-2的肺炎克雷伯菌临床分离株，它们与大流行菌株ST258相对应。这些分离株对头孢菌素、碳青霉烯类、喹诺酮类和黏菌素表现出高度耐药性。将产生KPC-2的肺炎克雷伯菌分离株与先前研究的来自墨西哥一次暴发的产生KPC-3的肺炎克雷伯菌分离株进行比较；它们显示出不相关的脉冲场凝胶电泳指纹图谱模式和不同的质粒图谱。KPC-2碳青霉烯酶基因在两个230 kb和270 kb的非接合性质粒中被鉴定出来；然而，1株分离株将KPC-2基因转移到了一个80 kb的质粒上。这些发现支持了在墨西哥医院对耐碳青霉烯类分离株进行持续分子流行病学监测的必要性。
