[STAT3、p-STAT3和E-钙黏蛋白在结直肠癌中的表达及临床意义]

[Expressions of STAT3, p-STAT3 and E-cadherin in colorectal cancer and clinical implications].

作者信息

Zhong Baoyuan, Liu Qingquan, Liu Yanxiu, Xiong Xiaoliang, Liu Yao

机构信息

Department of General Surgery, The First Affiliated Hospital, Gannan Medical College, Jiangxi Ganzhou 341000, China.

出版信息

Zhonghua Wei Chang Wai Ke Za Zhi. 2014 Jun;17(6):594-7.

PMID:24953370

Abstract

OBJECTIVE

To investigate the correlation of expressions of STAT3 and p-STAT3 with epithelial mesenchymal transition(EMT)-associated protein E-cadherin in colorectal cancer, and to examine the association of above expressions with tumor invasion and metastasis of colorectal cancer.

METHODS

Immunohistochemistry assay ElivisionTM plus was used to detect the expressions of STAT3, p-STAT3 and E-cadherin protein in colorectal cancer tissue samples of 50 cases and their corresponding adjacent non-tumor tissues. Association of these protein expressions with tumor invasion and metastasis was analyzed with χ(2) test. Correlation of STAT3 and p-STAT3 with E-cadherin was analyzed with Spearman method.

RESULTS

Positive expression rates of STAT3, p-STAT3 and E-cadherin protein in colorectal cancer tissues were 72%(36/50), 76%(38/50) and 26%(13/50), which were significantly higher compared to adjacent normal intestinal mucosa tissues [24%(12/50), 26%(13/50) and 68%(34/50), all P<0.05]. STAT3, p-STAT3 and E-cadherin expressions were associated with tumor differentiation, tumor invasion depth, tumor size, lymph node metastasis, TNM staging (all P<0.05). In colorectal cancer tissues, STAT3 protein expression was positively correlated with p-STAT3 expression. STAT3 and p-STAT3 expressions in colorectal cancer tissues were negatively correlated with E-cadherin expression(P<0.05).

CONCLUSION

STAT3 and p-STAT3 may be involved in tumor EMT through inhibition of E-cadherin expression, leading to the development of colorectal cancer.

摘要

目的

探讨信号转导与转录激活因子3（STAT3）及磷酸化信号转导与转录激活因子3（p-STAT3）的表达与结直肠癌上皮间质转化（EMT）相关蛋白E-钙黏蛋白的相关性，并检测上述表达与结直肠癌肿瘤侵袭和转移的关系。

方法

采用免疫组织化学ElivisionTM plus法检测50例结直肠癌组织样本及其相应癌旁非肿瘤组织中STAT3、p-STAT3和E-钙黏蛋白的表达。采用χ²检验分析这些蛋白表达与肿瘤侵袭和转移的关系。采用Spearman法分析STAT3和p-STAT3与E-钙黏蛋白的相关性。

结果

结直肠癌组织中STAT3、p-STAT3和E-钙黏蛋白的阳性表达率分别为72%（36/50）、76%（38/50）和26%（13/50），显著高于癌旁正常肠黏膜组织[24%（12/50）、26%（13/50）和68%（34/50），均P<0.05]。STAT3、p-STAT3和E-钙黏蛋白的表达与肿瘤分化、肿瘤浸润深度、肿瘤大小、淋巴结转移、TNM分期均相关（均P<0.05）。在结直肠癌组织中，STAT3蛋白表达与p-STAT3表达呈正相关。结直肠癌组织中STAT3和p-STAT3的表达与E-钙黏蛋白表达呈负相关（P<0.05）。