Zhao Yumiao, Xu Aiguo, Xu Qinfu, Zhao Wei, Li Dandan, Fang Xiang, Ren Yinlong
Critical Section, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan Province, China.
Department of Respiratory, Thoracic Hospital of Henan Province Zhengzhou 450052, Henan Province, China.
Int J Clin Exp Med. 2014 Apr 15;7(4):968-72. eCollection 2014.
To study the efficacy of bone marrow mesenchymal stem cell (MSC) transplantation to treat pulmonary emphysema in rats.
Sixty rats were randomly divided into control, model and transplantation groups. Each group contained 20 rats. Rat models of emphysema were established via intratracheal instillation of lipopolysaccharide and exposure of model and transplantation groups to smoke. Then, cultured bone marrow MSCs were injected into rats in the transplantation group via the tail vein. Pathological changes of the lung in rats were observed.
Emphysemic pathological changes were found in model and transplantation groups, but changes were significantly attenuated after transplantation, compared with that of the model group. The mean alveoli number (MAN) and pulmonary alveolar area (PAA) showed statistically significant differences among the three groups (P < 0.001). The MAN in the transplantation group was higher than that in the model group, but still lower than that in the control group. The PAA in the transplantation group was lower than that in the model group, but higher than that in the control group (P < 0.05). After transplantation, Brdu-positive cells were observed and CK was expressed in a small number of Brdu-positive cells. Brdu- and CK-positive cells were not found in control and model groups.
Transplantation of bone marrow MSCs can significantly attenuate lung inflammation and pathological changes in emphysemic rats, which may be associated with MSC differentiation into alveolar epithelial cells in recipient lung tissues.
研究骨髓间充质干细胞（MSC）移植治疗大鼠肺气肿的疗效。
将60只大鼠随机分为对照组、模型组和移植组，每组20只。通过气管内滴注脂多糖建立大鼠肺气肿模型，模型组和移植组大鼠暴露于烟雾中。然后，将培养的骨髓间充质干细胞经尾静脉注入移植组大鼠体内。观察大鼠肺组织的病理变化。
模型组和移植组均出现肺气肿病理改变，但移植后与模型组相比，病理改变明显减轻。三组间平均肺泡数（MAN）和肺泡面积（PAA）差异有统计学意义（P<0.001）。移植组的MAN高于模型组，但仍低于对照组。移植组的PAA低于模型组，但高于对照组（P<0.05）。移植后观察到Brdu阳性细胞，少数Brdu阳性细胞表达CK。对照组和模型组未发现Brdu和CK双阳性细胞。
骨髓间充质干细胞移植可显著减轻肺气肿大鼠的肺部炎症和病理改变，这可能与骨髓间充质干细胞在受体肺组织中分化为肺泡上皮细胞有关。
