Chatterjee Kaushik, Hung Stevephen, Kumar Girish, Simon Carl G
Polymers Division, National Institute of Standards & Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA.
J Funct Biomater. 2012 May 22;3(2):372-81. doi: 10.3390/jfb3020372.
Protein adsorption is known to direct biological response to biomaterials and is important in determining cellular response in tissue scaffolds. In this study we investigated the effect of the duration of protein adsorption to 3D polymer scaffolds on cell attachment and proliferation. 3D macro-porous polymer scaffolds were pre-aged in serum-containing culture medium for 5 min, 1 d or 7 d prior to seeding osteoblasts. The total amount of protein adsorbed was found to increase with pre-ageing time. Cell attachment and proliferation were measured 1 d and 14 d, respectively, after cell seeding. Osteoblast proliferation, but not attachment, increased with scaffold pre-ageing time and amount of adsorbed serum protein. These results demonstrate that the amount of time that scaffolds are exposed to serum-containing medium can affect cell proliferation and suggest that these effects are mediated by differences in the amount of protein adsorption.
众所周知，蛋白质吸附可引导对生物材料的生物学反应，并且在确定组织支架中的细胞反应方面很重要。在本研究中，我们研究了蛋白质吸附到三维聚合物支架上的持续时间对细胞附着和增殖的影响。在接种成骨细胞之前，将三维大孔聚合物支架在含血清的培养基中预老化5分钟、1天或7天。发现吸附的蛋白质总量随着预老化时间的增加而增加。在细胞接种后分别于第1天和第14天测量细胞附着和增殖情况。成骨细胞增殖而非附着，随着支架预老化时间和吸附血清蛋白量的增加而增加。这些结果表明，支架暴露于含血清培养基中的时间量可影响细胞增殖，并表明这些影响是由蛋白质吸附量的差异介导的。
