Department of Nanobiochemistry, Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.
ACS Appl Mater Interfaces. 2022 Aug 3;14(30):34443-34454. doi: 10.1021/acsami.2c08677. Epub 2022 Jul 20.
Metal-organic frameworks (MOFs) are porous materials with adsorption, storage, and separation capabilities due to their high specific surface areas and large pore volumes. MOFs are thus used in biomedical applications, and MOF nanoparticles have been widely studied as nanocarriers for drug delivery systems. Several research groups recently reported that specific MOF nanoparticles can adsorb and retain proteins, suggesting to us that MOF nanoparticles may have advantages as novel cell culture scaffolds. However, MOF nanoparticles cannot be used as two-dimensional scaffolds for cells. We therefore established a bottom-up technique to construct two-dimensional MOFs [MIL-53 (Al)] on polymer films. The developed two-dimensional MIL-53 (Al) film [fMIL-53 (Al)] exhibited high serum protein adsorption, retention, and replenishment capabilities as compared to conventional cell culture scaffolds. β-Galactosidase, used as a model protein, adsorbed on fMIL-53 (Al) exhibited original enzymatic activity, indicating that proteins are not denatured during the adsorption process. The viability of mouse myoblast cells (C2C12) cultured on fMIL-53 (Al) was 100%, indicating the cell compatibility of fMIL-53 (Al). Importantly, C2C12 cells cultured on serum protein-preadsorbed fMIL-53 (Al) exhibited excellent long-term adhesion, morphology, and proliferation even in a medium lacking serum proteins, demonstrating an important advantage of fMIL-53 (Al) as a cell culture scaffold, given that conventional cell culture scaffolds typically require a serum-containing medium to support stable cell adhesion and proliferation. To our knowledge, this is the first report regarding the application of MOFs as cell culture scaffolds and will serve as a starting point for studying two- and three-dimensional MOF-based cellular scaffolds for cell culture systems and for and tissue engineering.
金属有机骨架（MOFs）由于其高比表面积和大孔体积而具有吸附、存储和分离能力，是一种多孔材料。MOFs 因此被应用于生物医学领域，MOF 纳米粒子作为药物传递系统的纳米载体得到了广泛的研究。最近有几个研究小组报道，特定的 MOF 纳米粒子可以吸附和保留蛋白质，这使我们认为 MOF 纳米粒子作为新型细胞培养支架可能具有优势。然而，MOF 纳米粒子不能作为细胞的二维支架。因此，我们建立了一种自下而上的技术，在聚合物薄膜上构建二维 MOFs [MIL-53(Al)]。与传统的细胞培养支架相比，所开发的二维 MIL-53(Al)薄膜[fMIL-53(Al)]表现出高的血清蛋白吸附、保留和补充能力。β-半乳糖苷酶作为模型蛋白，吸附在 fMIL-53(Al)上时表现出原始的酶活性，表明在吸附过程中蛋白质没有变性。在 fMIL-53(Al)上培养的小鼠成肌细胞（C2C12）的活力为 100%，表明 fMIL-53(Al)具有细胞相容性。重要的是，在预吸附血清蛋白的 fMIL-53(Al)上培养的 C2C12 细胞即使在缺乏血清蛋白的培养基中也表现出优异的长期粘附、形态和增殖能力，这表明 fMIL-53(Al)作为细胞培养支架具有重要优势，因为传统的细胞培养支架通常需要含血清的培养基来支持稳定的细胞粘附和增殖。据我们所知，这是关于将 MOFs 应用于细胞培养支架的首次报道，它将为研究二维和三维基于 MOF 的细胞支架在细胞培养系统和组织工程中的应用提供一个起点。
