Su Nana, Wu Qi, Liu Yuanyuan, Cai Jiangtao, Shen Wenbiao, Xia Kai, Cui Jin
College of Life Sciences, Nanjing Agricultural University , Nanjing 210095, Jiangsu, P. R. China.
J Agric Food Chem. 2014 Jul 9;62(27):6454-62. doi: 10.1021/jf5019593. Epub 2014 Jun 30.
The aims of the study were to investigate whether hydrogen gas (H2) was involved in regulation of anthocyanin biosynthesis in two contrasting radish (Raphanus sativus L.) varieties (low [LA] and high [HA] level of anthocyanin) under UV irradiation. The results showed that hydrogen-rich water (HRW) significantly blocked the UV-A-induced increase of H2O2 and O2(•-) accumulation, and enhanced the UV-A-induced increase of superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities in LA and HA. Furthermore, UV-A-induced increase of anthocyanin and total phenols was further enhanced only in HA sprouts cotreated with HRW. LC-MS/MS analysis showed that five anthocyanidins existed in HA sprouts, but only two in LA sprouts. Meanwhile, the cyanidin was the most abundant anthocyanidin in HA, and the cyanidin was 2-fold higher cotreated with HRW than UV-A. Molecular analyses showed that the anthocyanin biosynthesis-related genes were upregulated significantly in both HA (in particular) and LA sprouts treated with HRW plus UV-A. These data imply that HRW reestablishes reactive oxygen species homeostasis in both LA and HA, but exerts different effects on anthocyanin accumulation between them under UV-A.
本研究的目的是调查在紫外线照射下，氢气（H₂）是否参与调控两种花青素含量差异显著的萝卜（Raphanus sativus L.）品种（低花青素含量[LA]和高花青素含量[HA]）中花青素的生物合成。结果表明，富氢水（HRW）显著抑制了UV-A诱导的H₂O₂和O₂(•-)积累增加，并增强了UV-A诱导的LA和HA中超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）活性的增加。此外，仅在与HRW共同处理的HA芽苗菜中，UV-A诱导的花青素和总酚含量增加进一步增强。LC-MS/MS分析表明，HA芽苗菜中存在五种花青素，但LA芽苗菜中仅存在两种。同时，矢车菊素是HA中含量最丰富的花青素，与UV-A处理相比，与HRW共同处理时矢车菊素含量高出2倍。分子分析表明，在HRW加UV-A处理的HA（尤其是）和LA芽苗菜中，花青素生物合成相关基因均显著上调。这些数据表明，HRW在LA和HA中均能重建活性氧稳态，但在UV-A条件下，其对二者花青素积累的影响不同。
