Wackers Gideon, Vandenryt Thijs, Cornelis Peter, Kellens Evelien, Thoelen Ronald, De Ceuninck Ward, Losada-Pérez Patricia, van Grinsven Bart, Peeters Marloes, Wagner Patrick
Institute for Materials Research, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
Sensors (Basel). 2014 Jun 20;14(6):11016-30. doi: 10.3390/s140611016.
In this work we present the first steps towards a molecularly imprinted polymer (MIP)-based biomimetic sensor array for the detection of small organic molecules via the heat-transfer method (HTM). HTM relies on the change in thermal resistance upon binding of the target molecule to the MIP-type receptor. A flow-through sensor cell was developed, which is segmented into four quadrants with a volume of 2.5 μL each, allowing four measurements to be done simultaneously on a single substrate. Verification measurements were conducted, in which all quadrants received a uniform treatment and all four channels exhibited a similar response. Subsequently, measurements were performed in quadrants, which were functionalized with different MIP particles. Each of these quadrants was exposed to the same buffer solution, spiked with different molecules, according to the MIP under analysis. With the flow cell design we could discriminate between similar small organic molecules and observed no significant cross-selectivity. Therefore, the MIP array sensor platform with HTM as a readout technique, has the potential to become a low-cost analysis tool for bioanalytical applications.
在这项工作中，我们展示了迈向基于分子印迹聚合物（MIP）的仿生传感器阵列的第一步，该阵列通过热传递方法（HTM）检测小分子有机化合物。HTM依赖于目标分子与MIP型受体结合时热阻的变化。我们开发了一种流通式传感器池，它被分成四个象限，每个象限的体积为2.5 μL，允许在单个基板上同时进行四次测量。进行了验证测量，其中所有象限都接受了统一处理，并且所有四个通道都表现出相似的响应。随后，在使用不同MIP颗粒功能化的象限中进行测量。根据所分析的MIP，这些象限中的每一个都暴露于添加了不同分子的相同缓冲溶液中。通过流通池设计，我们可以区分相似的小分子有机化合物，并且未观察到明显的交叉选择性。因此，以HTM作为读出技术的MIP阵列传感器平台有潜力成为生物分析应用的低成本分析工具。
