College of Chemistry, Jilin University, Changchun 130012, China.
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Biosens Bioelectron. 2016 Mar 15;77:393-9. doi: 10.1016/j.bios.2015.09.060. Epub 2015 Sep 28.
The utility of molecularly imprinted polymer (MIP) as electrochemical sensor often suffers from its limited catalytic efficiency. Here, we proposed an alternative approach by combining the concept of MIP with the use of mimetic enzyme. A metronidazole imprinted polymer with nitroreductase-like activity was successfully achieved via an electrochemical method, where melamine served two purposes: functional monomer of MIP and component of mimetic enzyme. During the imprinting process, the redox-active center, which is responsible for catalysis, was introduced into the imprinted cavities. Accordingly, the imprinted polymer, having both catalysis centers and recognition sites, exhibited enhanced electrocatalytic activity and selectivity. The sensing performances of this metronidazole imprinted biomimetic sensor were evaluated in detail. Results revealed that the response to metronidazole was linear in the concentration range of 0.5-1000 μM, and the detection limit was 0.12 μM (S/N=3). In addition, we applied the proposed sensor to detect metronidazole in an injection solution and the results implied its feasibility for practical application.
分子印迹聚合物（MIP）作为电化学传感器的应用常常受到其催化效率有限的影响。在这里，我们提出了一种替代方法，即将 MIP 的概念与模拟酶的使用相结合。通过电化学方法成功制备了具有硝基还原酶样活性的甲硝唑印迹聚合物，其中三聚氰胺有两个作用：MIP 的功能单体和模拟酶的组成部分。在印迹过程中，负责催化的氧化还原活性中心被引入印迹腔中。因此，具有催化中心和识别位点的印迹聚合物表现出增强的电催化活性和选择性。详细评估了这种甲硝唑印迹仿生传感器的传感性能。结果表明，对甲硝唑的响应在 0.5-1000 μM 的浓度范围内呈线性，检测限为 0.12 μM（S/N=3）。此外，我们将所提出的传感器应用于注射液中甲硝唑的检测，结果表明其在实际应用中的可行性。
