Potecă T, Potecă A, Sajin M, Comănescu M
Chirurgia (Bucur). 2014 May-Jun;109(3):347-54.
Gastric cancer is the second leading cause of cancer mortality worldwide. (1) Gastric carcinogenesis involves a variety of factors including diet, habitual factors as well as environmental factors. (2,3) This study aimed to correlate clinicopathological parameters of the cases studied and PCNA and p53 expression using immunohistochemistry. The study group included a total of 32 patients that underwent gastrectomy for gastric cancer.The study parameters were represented by epidemiological aspects (age, sex), clinical characteristics (signs and symptoms),histopathological findings (pTNM staging and degree of differentiation, histological classification, lymph nodes status and presence of vascular invasion) and survival, and immunohistochemical analysis (p53 and PCNA expression) of the study group. Histopathological study showed that most of the cases(26 cases) were of the intestinal type and 6 cases of the diffuse type. Immunohistochemical analysis of p53 protein expression showed an average of 20.75% positive cells, while PCNA expression showed an average of 47.3%. In terms of survival there were 6 cases of death at intervals ranged from 2-189 days,5 cases had subsequent presentations over 12 months, while 8 patients were lost to follow-up. At the time of surgery, 6 patients had distant metastases, while 6 more developed them in a period of 2-12 months after surgery. Identification of biomolecules that highlight potentially aggressive tumors may help modulate the therapeutic approach after surgical resection.
胃癌是全球癌症死亡的第二大主要原因。（1）胃癌的发生涉及多种因素，包括饮食、习惯因素以及环境因素。（2,3）本研究旨在通过免疫组织化学将所研究病例的临床病理参数与PCNA和p53表达进行关联。研究组共纳入32例因胃癌接受胃切除术的患者。研究参数包括流行病学方面（年龄、性别）、临床特征（体征和症状）、组织病理学发现（pTNM分期和分化程度、组织学分类、淋巴结状态和血管侵犯情况）以及生存情况，还有研究组的免疫组织化学分析（p53和PCNA表达）。组织病理学研究表明，大多数病例（26例）为肠型，6例为弥漫型。p53蛋白表达的免疫组织化学分析显示平均阳性细胞率为20.75%，而PCNA表达显示平均为47.3%。在生存方面，有6例在2至189天期间死亡，5例在12个月后有后续表现，而8例患者失访。手术时，6例有远处转移，另有6例在术后2至12个月内出现远处转移。识别突出潜在侵袭性肿瘤的生物分子可能有助于调整手术切除后的治疗方法。
