Watson F, Robinson J J, Phelan M, Bucknall R C, Edwards S W
Department of Biochemistry, University of Liverpool, United Kingdom.
Ann Rheum Dis. 1993 May;52(5):354-9. doi: 10.1136/ard.52.5.354.
The aim of this study was to determine if neutrophils isolated from the blood and synovial fluid of patients with rheumatoid arthritis had patterns of receptor expression resembling those of blood neutrophils from controls which had been activated and primed in vitro.
Fluorescence activated cell sorting was used to measure receptor expression in paired blood and synovial fluid neutrophils from patients and in control neutrophils exposed to phorbol myristate acetate and granulocyte-macrophage colony stimulating factor.
There was no significant difference in the patterns of receptor expression in blood neutrophils from patients and healthy controls, but neutrophils in the synovial fluid had been primed and activated within the joint. About 50% of rheumatoid synovial fluid neutrophil samples expressed Fc gamma RI, a high affinity receptor for monomeric IgG, which is only expressed in neutrophils exposed to cytokines.
Synovial fluid neutrophils are activated and primed within the inflamed joint and hence their ability to respond to activating factors such as immune complexes will be modulated. As the expression of Fc gamma RI requires active biosynthesis, this work indicates that selective gene activation occurs when neutrophils are recruited into rheumatoid joints.
本研究旨在确定从类风湿性关节炎患者的血液和滑液中分离出的中性粒细胞是否具有与体外激活和致敏的对照血液中性粒细胞相似的受体表达模式。
采用荧光激活细胞分选技术来测量患者配对的血液和滑液中性粒细胞以及暴露于佛波酯肉豆蔻酸酯乙酸盐和粒细胞-巨噬细胞集落刺激因子的对照中性粒细胞中的受体表达。
患者血液中性粒细胞与健康对照的受体表达模式无显著差异，但滑液中的中性粒细胞已在关节内致敏和激活。约50%的类风湿性滑液中性粒细胞样本表达FcγRI，这是一种针对单体IgG的高亲和力受体，仅在暴露于细胞因子的中性粒细胞中表达。
滑液中性粒细胞在炎症关节内被激活和致敏，因此它们对诸如免疫复合物等激活因子的反应能力将受到调节。由于FcγRI的表达需要活跃的生物合成，这项研究表明当中性粒细胞被募集到类风湿关节时会发生选择性基因激活。
