Ichikawa Shunsuke, Abe Reimi, Fujimoto Haruka, Higashi Koushi, Zang Liqing, Nakayama Hiroko, Matsuoka Izumi, Shimada Yasuhito
Faculty of Education, Mie University, Tsu, Mie, Japan.
Mie University Zebrafish Drug Screening Center, Tsu, Japan.
Front Microbiol. 2023 Feb 16;14:1079187. doi: 10.3389/fmicb.2023.1079187. eCollection 2023.
Interventions to the gut microbiome manipulate the gut-brain axis and could be useful in the treatment of anxiety and depression. In this study, we demonstrated that administration of the bacterium reduces anxiety-like behavior in adult zebrafish. administration increased the diversity of the zebrafish gut microbiome. Linear discriminant analysis Effect Size (LEfSe) analysis revealed that the populations of Actinomycetales including Nocardiaceae, , Gordoniaceae, , Nakamurellaceae, and Aeromonadaceae were reduced, whereas those of Rhizobiales including Xanthobacteraceae, Bradyrhizobiaceae, Rhodospirillaceae, and Pirellulaceae were increased in the gut microbiome. Functional analysis using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) predicted that administration altered taurine metabolism in the zebrafish gut, and we demonstrated that administration increased the taurine concentration in the brain. Since taurine functions as an antidepressant neurotransmitter in vertebrates, our results suggest that could improve anxiety-like behavior in zebrafish the gut-brain axis.
对肠道微生物群的干预可调控肠-脑轴，可能有助于治疗焦虑和抑郁。在本研究中，我们证明给予这种细菌可减少成年斑马鱼的焦虑样行为。给予该细菌增加了斑马鱼肠道微生物群的多样性。线性判别分析效应大小（LEfSe）分析显示，肠道微生物群中包括诺卡氏菌科、戈登氏菌科、中井氏菌科和气单胞菌科在内的放线菌目菌群数量减少，而包括黄杆菌科、慢生根瘤菌科、红螺菌科和梨形菌科在内的根瘤菌目菌群数量增加。使用通过重建未观察状态进行群落系统发育研究（PICRUSt2）的功能分析预测，给予该细菌会改变斑马鱼肠道中的牛磺酸代谢，并且我们证明给予该细菌会增加大脑中的牛磺酸浓度。由于牛磺酸在脊椎动物中作为一种抗抑郁神经递质发挥作用，我们的结果表明给予该细菌可能通过肠-脑轴改善斑马鱼的焦虑样行为。
