INSERM UMR_1134, Paris, France Université Paris Diderot, Sorbonne Paris Cité, UMR_S1134, Paris, France Institut National de la Transfusion Sanguine, Paris, France Laboratory of Excellence GR-Ex, Paris, France.
Laboratory of Excellence GR-Ex, Paris, France Institut de Recherche pour le Développement, UMR_216, Mère et Enfant Face aux Infections Tropicales, Paris, France PRES Sorbonne Paris Cité, Faculté de Pharmacie, Paris, France.
Infect Immun. 2014 Sep;82(9):3783-9. doi: 10.1128/IAI.01922-14. Epub 2014 Jun 23.
Pregnancy-associated malaria (PAM) can lead to severe complications for both mother and baby. Certain placental cytokine/chemokine profiles have been shown to reflect poor pregnancy outcomes, including maternal anemia and low birth weight. In intervillous plasma samples from 400 Beninese women living in an area where Plasmodium falciparum is endemic, we quantified 16 cytokines/chemokines. We assessed their profiles in groups with PAM, with maternal anemia, with preterm births, or with a low birth weight for gestational age. Repeated ultrasound measurements ensured that prematurity and low birth weight were highly accurate. Preliminary analyses revealed trends for lower cytokine/chemokine concentrations in placental plasma associated both with babies with low birth weight for gestational age and with P. falciparum infection during pregnancy, while, as a function of the latter, the concentration of gamma interferon (IFN-γ)-inducible protein 10 (IP-10) was higher. Multivariate analyses showed that (i) higher placental plasma interleukin-10 (IL-10) levels were associated with P. falciparum infections and (ii) independently of P. falciparum infections, lower concentrations of both IFN-γ and IL-5 were associated with low birth weight for gestational age. Our data further strengthen the idea that IL-10 and IP-10 could be useful diagnostic markers of P. falciparum infection during pregnancy. The concentrations of cytokines/chemokines in placental plasma may represent previously unrecognized markers of poor fetal growth.
妊娠相关疟疾（PAM）可导致母婴严重并发症。某些胎盘细胞因子/趋化因子谱已被证明反映了不良妊娠结局，包括母体贫血和低出生体重。在来自 400 名贝宁妇女的绒毛间血浆样本中，这些妇女生活在恶性疟原虫流行的地区，我们定量了 16 种细胞因子/趋化因子。我们在患有 PAM、贫血、早产或足月低体重的组中评估了它们的特征。重复的超声测量确保了早产和低出生体重的高度准确性。初步分析显示，与足月低体重相关的胎盘血浆中细胞因子/趋化因子浓度呈下降趋势，与怀孕期间感染恶性疟原虫有关，而随着后者的发生，γ干扰素（IFN-γ）诱导的蛋白 10（IP-10）浓度升高。多变量分析表明：（i）较高的胎盘血浆白细胞介素-10（IL-10）水平与恶性疟原虫感染有关；（ii）独立于恶性疟原虫感染，IFN-γ和 IL-5 的浓度较低与足月低体重有关。我们的数据进一步加强了这样一种观点，即 IL-10 和 IP-10 可能是妊娠期间恶性疟原虫感染的有用诊断标志物。胎盘血浆中细胞因子/趋化因子的浓度可能代表了以前未被识别的胎儿生长不良的标志物。
