Lian Jiazhang, Zhao Huimin
†Department of Chemical and Biomolecular Engineering, ‡Energy Biosciences Institute, Institute for Genomic Biology, §Departments of Chemistry, Biochemistry, and Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
ACS Synth Biol. 2015 Mar 20;4(3):332-41. doi: 10.1021/sb500243c. Epub 2014 Jul 2.
Functionally reversing the β-oxidation cycle represents an efficient and versatile strategy for synthesis of a wide variety of fuels and chemicals. However, due to the compartmentalization of cellular metabolisms, reversing the β-oxidation cycle in eukaryotic systems remains elusive. Here, we report the first successful reversal of the β-oxidation cycle in Saccharomyces cerevisiae, an important cell factory for large-scale production of fuels and chemicals. After extensive gene cloning and enzyme activity assays, a reversed β-oxidation pathway was functionally constructed in the yeast cytosol, which led to the synthesis of n-butanol, medium-chain fatty acids (MCFAs), and medium-chain fatty acid ethyl esters (MCFAEEs). The resultant recombinant strain provides a new broadly applicable platform for synthesis of fuels and chemicals in S. cerevisiae.
从功能上逆转β-氧化循环是合成多种燃料和化学品的一种高效且通用的策略。然而，由于细胞代谢的区室化，在真核系统中逆转β-氧化循环仍然难以实现。在此，我们报告了在酿酒酵母（一种用于大规模生产燃料和化学品的重要细胞工厂）中首次成功逆转β-氧化循环。经过广泛的基因克隆和酶活性测定，在酵母细胞质中功能性地构建了一条逆转的β-氧化途径，该途径导致了正丁醇、中链脂肪酸（MCFAs）和中链脂肪酸乙酯（MCFAEEs）的合成。所得的重组菌株为酿酒酵母中燃料和化学品的合成提供了一个新的广泛适用的平台。
