Kasperczyk Sławomir, Dobrakowski Michał, Kasperczyk Aleksandra, Zalejska-Fiolka Jolanta, Pawlas Natalia, Kapka-Skrzypczak Lucyna, Birkner Ewa
Department of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, Katowice, Poland.
Laboratory of Genetic Toxicology, Institute of Occupational Medicine and Environmental Health, Sosnowiec, Poland 3 Department of Molecular Biology and Translational Research, Institute of Rural Health, Lublin, Poland.
Ann Agric Environ Med. 2014;21(2):272-7. doi: 10.5604/1232-1966.1108590.
There are no published studies examining the effects of N-acetylcysteine (NAC) administration on the non-enzymatic defence systems in humans exposed to lead. In view of this, it was decided to measure the levels of uric acid (UA), albumin, bilirubin and alpha-tocopherol before and after treatment with NAC. An estimation was also made of the degree of oxidative stress by measuring the ferric reducing ability of plasma (FRAP), the levels of conjugated dienes (CD) and lipid hydroperoxides (LHP). Male employees who worked with lead were randomized into two groups. The first group included workers who were not administered any drugs (n=49), while the second group (n=122) consisted of workers who were treated with NAC at three different doses (200 mg, 400 mg and 800 mg) for 12 weeks. The administration of NAC (400 mg, 800 mg) resulted in significant decreases in the LHP levels. Similarly, a strong tendency toward lower levels of CD was observed in the same groups. The UA levels were significantly lower only in the group receiving the 200 mg dose of NAC. However, the alpha-tocopherol levels were significantly elevated after treatment with NAC (400 mg, 800 mg). NAC administration did not significantly affect the levels of bilirubin and albumin, but a tendency toward higher values was observed for FRAP. NAC reduced the extent of lipid peroxidation in a dose-dependent manner. Elevated concentrations of alpha-tocopherol may have enhanced the beneficial effects of NAC. Treatment with NAC may contribute to the restoration of non-enzymatic antioxidant reserves when administered to lead-exposed workers.
目前尚无已发表的研究探讨给予N-乙酰半胱氨酸（NAC）对铅暴露人群非酶防御系统的影响。鉴于此，决定在使用NAC治疗前后测量尿酸（UA）、白蛋白、胆红素和α-生育酚的水平。还通过测量血浆铁还原能力（FRAP）、共轭二烯（CD）水平和脂质氢过氧化物（LHP）来评估氧化应激程度。从事铅作业的男性员工被随机分为两组。第一组包括未服用任何药物的工人（n = 49），而第二组（n = 122）由接受三种不同剂量（200 mg、400 mg和800 mg）NAC治疗12周的工人组成。给予NAC（400 mg、800 mg）导致LHP水平显著降低。同样，在同一组中观察到CD水平有明显降低的趋势。仅在接受200 mg剂量NAC的组中UA水平显著降低。然而，用NAC（400 mg、800 mg）治疗后α-生育酚水平显著升高。给予NAC对胆红素和白蛋白水平没有显著影响，但FRAP有升高的趋势。NAC以剂量依赖的方式降低脂质过氧化程度。α-生育酚浓度升高可能增强了NAC的有益作用。对铅暴露工人给予NAC治疗可能有助于恢复非酶抗氧化储备。
