Perin Luana Martins, Miranda Rodrigo Otávio, Todorov Svetoslav Dimitrov, Franco Bernadette Dora Gombossy de Melo, Nero Luís Augusto
Departamento de Veterinária, Universidade Federal de Viçosa, 36570 900 Viçosa, MG, Brazil.
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 05508 000 São Paulo, SP, Brazil.
Int J Food Microbiol. 2014 Aug 18;185:121-6. doi: 10.1016/j.ijfoodmicro.2014.06.001. Epub 2014 Jun 12.
The present study aimed to investigate the virulence, antibiotic resistance and biogenic amine production in bacteriocinogenic lactococci and enterococci isolated from goat milk in order to evaluate their safety. Twenty-nine bacteriocinogenic lactic acid bacteria (LAB: 11 Lactococcus spp., and 18 Enterococcus spp.) isolated from raw goat milk were selected and subjected to PCR to identify gelE, cylA, hyl, asa1, esp, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc genes. The expression of virulence factors (gelatinase, hemolysis, lipase, DNAse, tyramine, histamine, putrescine) in different incubation temperatures was assessed by phenotypic methods, as well as the resistance to vancomycin, gentamicin, chloramphenicol, ampicillin and rifampicin (using Etest®). The tested isolates presented distinct combinations of virulence related genes, but not necessarily the expression of such factors. The relevance of identifying virulence-related genes in bacteriocinogenic LAB was highlighted, demanding for care in their usage as starter cultures or biopreservatives due to the possibility of horizontal gene transfer to other bacteria in food systems.
本研究旨在调查从山羊奶中分离出的产细菌素的乳酸乳球菌和肠球菌的毒力、抗生素抗性及生物胺生成情况，以评估其安全性。从生山羊奶中分离出29株产细菌素的乳酸菌（LAB：11株乳酸乳球菌和18株肠球菌），并进行PCR以鉴定gelE、cylA、hyl、asa1、esp、efaA、ace、vanA、vanB、hdc1、hdc2、tdc和odc基因。通过表型方法评估不同培养温度下毒力因子（明胶酶、溶血、脂肪酶、DNA酶、酪胺、组胺、腐胺）的表达，以及对万古霉素、庆大霉素、氯霉素、氨苄青霉素和利福平的抗性（使用Etest®）。受试分离株呈现出不同的毒力相关基因组合，但不一定表达这些因子。强调了在产细菌素的LAB中鉴定毒力相关基因的重要性，鉴于其可能水平基因转移至食品系统中的其他细菌，因此在将其用作发酵剂培养物或生物防腐剂时需谨慎。
