Mendoza L, Marin G
Mycoses. 1989 Feb;32(2):73-7. doi: 10.1111/j.1439-0507.1989.tb02205.x.
Antigens and rabbit-antisera from holotypes of Pythium insidiosum and P. destruens were prepared to elucidate their antigenic relationship. The antigens and rabbit-antisera of P. insidiosum as well as P. destruens used as a reference system showed that both shared three precipitin bands in common. The antigen and rabbit-antisera of P. destruens and P. insidiosum used as a reference system against other strains isolated from humans and animals with pythiosis, also showed three precipitin bands in common. When we used sera taken from horses with proven pythiosis against antigens of P. insidiosum and P. destruens, six common bands were observed. We concluded that the etiologic agent of pythiosis is a single species P. insidiosum, and could be identified by serologic methods.
制备了来自毁灭腐霉和隐匿腐霉全模式标本的抗原及兔抗血清,以阐明它们的抗原关系。隐匿腐霉和毁灭腐霉的抗原和兔抗血清作为参考系统显示,二者共有三条沉淀带。毁灭腐霉和隐匿腐霉的抗原及兔抗血清作为参考系统,用于检测从患有腐霉病的人和动物中分离出的其他菌株时,也显示出三条共有沉淀带。当我们使用来自确诊患有腐霉病的马的血清检测隐匿腐霉和毁灭腐霉的抗原时,观察到六条共有带。我们得出结论,腐霉病的病原体是单一物种隐匿腐霉,并且可以通过血清学方法进行鉴定。