Mendoza L, Kaufman L, Standard P G
J Clin Microbiol. 1986 May;23(5):813-6. doi: 10.1128/jcm.23.5.813-816.1986.
A practical, sensitive, and specific immunodiffusion test was developed for diagnosing and monitoring pythiosis in horses. Culture filtrates, a soluble cell mass, and trypsinized Pythium sp. antigens were evaluated against prepared rabbit anti-Pythium sp. serum and pythiosis horse case sera. The culture filtrate antigens demonstrated the greatest capacity for detecting precipitins and the greatest stability during storage. In contrast, the trypsinized antigens had the weakest capability for detecting multiple precipitins and the poorest stability. The 13 sera from horses with proven active pythiosis were positive in immunodiffusion tests with the culture filtrate antigens. Each serum contained from three to six precipitins. Treated horses lost precipitins, and some became antibody negative. No false-positive reactions were noted in tests with sera from normal horses and humans or with sera from a variety of heterologous horse and human infections.
开发了一种实用、灵敏且特异的免疫扩散试验，用于诊断和监测马的芽生菌病。针对制备的兔抗芽生菌属血清和芽生菌病马病例血清，对培养滤液、可溶性细胞团和胰蛋白酶处理的芽生菌属抗原进行了评估。培养滤液抗原在检测沉淀素方面表现出最大能力，且在储存期间稳定性最高。相比之下，胰蛋白酶处理的抗原检测多种沉淀素的能力最弱，稳定性最差。13份经证实患有活动性芽生菌病的马血清在与培养滤液抗原的免疫扩散试验中呈阳性。每份血清含有三到六种沉淀素。接受治疗的马沉淀素减少，一些马变为抗体阴性。在使用正常马和人的血清或各种异源性马和人感染血清进行的试验中未观察到假阳性反应。
