3种根管封闭剂对龋齿放线菌的体外抗菌作用
In vitro antimicrobial effects of 3 root canal sealers on Actinomyces radicidentis.
作者信息
Barbero-Navarro Ignacio, Galera-Ruiz Hugo, Pereira Mario, Guerreiro Diogo, Machuca-Portillo María del Carmen, López del Valle Lydia
出版信息
P R Health Sci J. 2014 Jun;33(2):71-3.
OBJECTIVE
To determine the in vitro antimicrobial effects of 3 endodontic sealers--AH Plus, Sealapex, and Tubli-Seal--on Actinomyces radicidentis, a bacterial species commonly found in root canals.
METHODS
Prior to the experimental procedures, bacterial identification tests, such as Gram staining, catalase, and API 20A, were performed, and the bacteria were identified as A. radicidentis. The agar diffusion susceptibility test was performed to determine the areas of bacterial growth inhibition and, consequently, the microbial resistance of the 3 sealers against A. radicidentis. Chlorhexidine was used as a positive control, and saline solution was used as a negative control.
RESULTS
Tubli-Seal cement had an average diameter of inhibition zones in the 3 panels of 22.73 mm, that of AH Plus was 17.13 mm, and that of Sealapex, 11.99 mm. A one-way ANOVA test showed that there were significant differences between the 3 cements (p < 0.05).
CONCLUSION
Tubli-Seal showed the highest levels of antimicrobial activity, which was followed by AH Plus with the next highest levels and, finally, Sealapex with the lowest levels of antimicrobial activity.
目的
确定三种根管封闭剂——AH Plus、Sealapex和Tubli-Seal——对根管中常见的细菌物种齿放线菌的体外抗菌效果。
方法
在实验步骤之前，进行了细菌鉴定试验，如革兰氏染色、过氧化氢酶试验和API 20A试验，鉴定该细菌为齿放线菌。采用琼脂扩散药敏试验来确定细菌生长抑制区域，从而确定这三种封闭剂对齿放线菌的微生物耐药性。洗必泰用作阳性对照，盐溶液用作阴性对照。
结果
Tubli-Seal水门汀在3个平板中的抑菌圈平均直径为22.73mm，AH Plus为17.13mm，Sealapex为11.99mm。单向方差分析显示，这三种水门汀之间存在显著差异（p < 0.05）。
结论
Tubli-Seal表现出最高水平的抗菌活性，其次是AH Plus，抗菌活性水平次之，最后是Sealapex，抗菌活性水平最低。
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