Guo Peir-Fen, Chen Tina Tu-Wen, Tsaihong John Chin, Ho Gen-Der, Cheng Po-Ching, Tseng Yu-Chuan, Peng Shih-Yi
Southeast Asian J Trop Med Public Health. 2014 Jan;45(1):6-12.
Cryptosporidiosis is a zoonotic disease caused by the protozoan parasite Cryptosporidium. A total of 436 horse fecal samples were collected from 19 farms, and acid-fast staining method was used for primary screening. Cryptosporidium oocysts were found in 161 samples, among which 33 positive sample were selected for nested PCR, restriction fragment length polymorphism analysis and DNA sequencing of 18 S rDNA, showing 31 samples to be bovine C. parvum and 2 C. felis. The methods employed in this study should be useful as tools to identify cryptosporidiosis genotypes and species of livestock.
隐孢子虫病是一种由原生动物寄生虫隐孢子虫引起的人畜共患病。从19个农场共采集了436份马粪便样本，并采用抗酸染色法进行初步筛查。在161份样本中发现了隐孢子虫卵囊，其中33份阳性样本被选用于巢式PCR、限制性片段长度多态性分析和18S rDNA的DNA测序，结果显示31份样本为牛微小隐孢子虫，2份为猫隐孢子虫。本研究中采用的方法应可作为鉴定家畜隐孢子虫病基因型和种类的工具。
